Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/11

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(New page: ===1:PCR=== <Member><br /> mio,easily <Sample><br /> *2 <Protocol><br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPO...)
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Revision as of 07:07, 27 September 2010

Contents

1:PCR

<Member>
mio,easily


<Sample>

  • 2


<Protocol>
See Protocol 2

(temperature in annealing)

  • 2:70.0℃


2:Digestion(1)

<Member>
Mariko,mizuki


<Sample>

  • 1H:Avr2,FD Buffer
  • 2(9/6):Nhe1、M Buffer
  • 4(8/30):Avr2,FD Buffer
  • 5(8/30):Spe1、M Buffer


<Protocol>
See Prptpcpl 9


3:Electrophoresis

<Member>
Mariko,mio


<Sample>

  • PCR products(9/11)
  • Digestion products(9/11)


<Protocol>
See Protocol 8


4:DNA extraction

<Member>
mizuki,easily


<Sample>
PCR products(9/11)


<Protocol>
See Protocol 4


5:Digestion(2)

<Member>
easily


<Sample>

  • 1(9/11)


<Protocol>
See Prptpcpl 9


6:Electrophoresis

<Member>
Mariko,mizuki


<Sample>
Digestion products

  • 1(9/11)
  • 4L(8/26)
  • 8H


<Protocol>
See Protocol 8


8:DNA extraction

<Member>
mio,mizuki


<Sample>

  • 4(9/11)
  • 5(9/11)


<Protocol>
See Protocol 4


9:Ligation

<Member>
Mariko,mio


<Sample>

  • 4(9/11)
  • 5(9/11)


<Protocol>
See Protocol 10


10:DNA extraction

<Member>
Mariko,mio,mizuki


<Sample>

  • 1(9/11)
  • 2(9/11)


<Protocol>
See Protocol 4


12:Ligation

<Member>
Mariko


<Sample>

  • 1(9/11)
  • 2(9/11)


<Protocol>
See Protocol 10

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/11"