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Team:Osaka/Protocols
From 2010.igem.org
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*Transformation by heat-shock | *Transformation by heat-shock | ||
*Plasmid DNA miniprep | *Plasmid DNA miniprep | ||
| - | * 3A BioBrick assembly | + | *3A BioBrick assembly |
*PCR cloning | *PCR cloning | ||
*PCR site-directed mutagenesis | *PCR site-directed mutagenesis | ||
*Extraction of genome DNA for PCR cloning | *Extraction of genome DNA for PCR cloning | ||
Revision as of 12:33, 25 September 2010
| Home | Team | Project | Submitted Parts | Modeling | Protocols | Notebook | Safety |
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Protocols
- Preparation of competent cells for heat-shock transformation
- Transformation by heat-shock
- Plasmid DNA miniprep
- 3A BioBrick assembly
- PCR cloning
- PCR site-directed mutagenesis
- Extraction of genome DNA for PCR cloning
