Team:Osaka/Protocols
From 2010.igem.org
(Difference between revisions)
(→Protocols) |
(→Protocols) |
||
Line 13: | Line 13: | ||
==Protocols== | ==Protocols== | ||
- | + | *Preparation of competent cells for heat-shock transformation | |
- | + | *Transformation by heat-shock | |
- | + | *Plasmid DNA miniprep | |
- | + | * 3A BioBrick assembly | |
- | + | *PCR cloning | |
- | + | *PCR site-directed mutagenesis | |
- | + | *Extraction of genome DNA for PCR cloning |
Revision as of 12:33, 25 September 2010
Home | Team | Project | Submitted Parts | Modeling | Protocols | Notebook | Safety |
---|
Protocols
- Preparation of competent cells for heat-shock transformation
- Transformation by heat-shock
- Plasmid DNA miniprep
- 3A BioBrick assembly
- PCR cloning
- PCR site-directed mutagenesis
- Extraction of genome DNA for PCR cloning