Team:Osaka/Protocols

From 2010.igem.org

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(Protocols)
(Protocols)
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==Protocols==
==Protocols==
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# Preparation of competent cells for heat-shock transformation
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*Preparation of competent cells for heat-shock transformation
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# Transformation by heat-shock
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*Transformation by heat-shock
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# Plasmid DNA miniprep
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*Plasmid DNA miniprep
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# 3A BioBrick assembly
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* 3A BioBrick assembly
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# PCR cloning
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*PCR cloning
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# PCR site-directed mutagenesis
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*PCR site-directed mutagenesis
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# Extraction of genome DNA for PCR cloning
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*Extraction of genome DNA for PCR cloning

Revision as of 12:33, 25 September 2010

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Protocols

  • Preparation of competent cells for heat-shock transformation
  • Transformation by heat-shock
  • Plasmid DNA miniprep
  • 3A BioBrick assembly
  • PCR cloning
  • PCR site-directed mutagenesis
  • Extraction of genome DNA for PCR cloning