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Team:Calgary/9 June 2010
From 2010.igem.org
(Difference between revisions)
(New page: Himika and Emily: Miniprep B0034 and B0015, Colony PCR of construct of B0034 and E1010. Dev, Chris, and Jeremy Alex and Patrick) |
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| - | Himika and Emily: | + | Himika and Emily: Today, they Miniprepped the overnight cultures of B0034 and B0015 which were made yesterday. A colony PCR of construct of B0034 and E1010 was done as well to determine if the parts were in fact correctly attached together. |
| - | Dev, Chris, and Jeremy | + | Dev, Chris, and Jeremy: Today, they Miniprepped the plasmid backbones pSB1A2, pSB1AC3, pSB1AK3, and pSB1K3 so they could be used in a plasmid transfer. Then, they made constructs to put the parts E0032 and E0040 into the kanamycin plasmids which could then be grown overnight. |
Alex and Patrick | Alex and Patrick | ||
Revision as of 20:38, 9 June 2010
Himika and Emily: Today, they Miniprepped the overnight cultures of B0034 and B0015 which were made yesterday. A colony PCR of construct of B0034 and E1010 was done as well to determine if the parts were in fact correctly attached together.
Dev, Chris, and Jeremy: Today, they Miniprepped the plasmid backbones pSB1A2, pSB1AC3, pSB1AK3, and pSB1K3 so they could be used in a plasmid transfer. Then, they made constructs to put the parts E0032 and E0040 into the kanamycin plasmids which could then be grown overnight.
Alex and Patrick
