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Team:UT-Tokyo/Protocols
From 2010.igem.org
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Revision as of 06:01, 23 September 2010
Protocols
Notice
-You should mix reagents well -Put in enzymes after you mixed all other reagents -Centrifuge the tube and collect substances on the bottom before opening a reagent
Transformation Colony Pick Up Miniprep Restriction Enzyme Transaction（Xba1/Pst1）—Once for all Restriction Enzyme Transaction —One by one PCR（Pfu Ultra） PCR（Ex-Taq） PCR（KODplus） Colony PCR（Ex-Taq） Electrophoresis Gel DNA Recovery LB broth Agarose Gel Seaquencing Producing Competent Cell
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