Team:UT-Tokyo/Protocols

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
__NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}}
__NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}}
 +
<h2>Protocols</h2>
-
! Notice !
+
 
 +
  Notice  
-You should mix reagents well
-You should mix reagents well
-Put in enzymes after you mixed all other reagents
-Put in enzymes after you mixed all other reagents

Revision as of 05:58, 23 September 2010

UT-Tokyo

Protocols


 Notice 

-You should mix reagents well -Put in enzymes after you mixed all other reagents -Centrifuge the tube and collect substances on the bottom before opening a reagent


Transformation Colony Pick Up Miniprep Restriction Enzyme Transaction(Xba1/Pst1)—Once for all Restriction Enzyme Transaction —One by one PCR(Pfu Ultra) PCR(Ex-Taq) PCR(KODplus) Colony PCR(Ex-Taq) Electrophoresis Gel DNA Recovery LB broth Agarose Gel Seaquencing Producing Competent Cell