Team:UT-Tokyo/Protocols
From 2010.igem.org
(Difference between revisions)
Line 1: | Line 1: | ||
__NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} | __NOTOC__{{UT-Tokyo_CSS3}}{{UT-Tokyo_Head}} | ||
+ | <h2>Protocols</h2> | ||
- | + | ||
+ | Notice | ||
-You should mix reagents well | -You should mix reagents well | ||
-Put in enzymes after you mixed all other reagents | -Put in enzymes after you mixed all other reagents |
Revision as of 05:58, 23 September 2010
Protocols
Notice
-You should mix reagents well -Put in enzymes after you mixed all other reagents -Centrifuge the tube and collect substances on the bottom before opening a reagent
Transformation Colony Pick Up Miniprep Restriction Enzyme Transaction(Xba1/Pst1)—Once for all Restriction Enzyme Transaction —One by one PCR(Pfu Ultra) PCR(Ex-Taq) PCR(KODplus) Colony PCR(Ex-Taq) Electrophoresis Gel DNA Recovery LB broth Agarose Gel Seaquencing Producing Competent Cell
Copyright © 2010 iGEM UT-Tokyo. All rights reserved.