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Team:USTC/Notebook/Meetings/15 September 2010
From 2010.igem.org
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Add 1mM IPTG in bacteria in LB to induce protein production in 16℃、30℃. | Add 1mM IPTG in bacteria in LB to induce protein production in 16℃、30℃. | ||
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| + | ---- | ||
| + | Hilde: | ||
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| + | PCR test of pduN (N-r, N-f) Model: NTUV plm.-1/-2/-3; NTUV plm.2 No.1-2-3-4; ABJKNTUV#2-#9-#5;TUV5(AD);NULL | ||
Revision as of 09:40, 22 September 2010
Jocelyn:
Double Digest of pduA, pduB in BBF RFC 53 manner. => failed in A...T.T => Digest pduA again.
Ligation of pduJK (solutino I & solution I+II)、pduNU、plasimd with RBS.
Add 1mM IPTG in bacteria in LB to induce protein production in 16℃、30℃.
Hilde:
PCR test of pduN (N-r, N-f) Model: NTUV plm.-1/-2/-3; NTUV plm.2 No.1-2-3-4; ABJKNTUV#2-#9-#5;TUV5(AD);NULL
