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Team:USTC/Notebook/Meetings/11 September 2010
From 2010.igem.org
(Difference between revisions)
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Double Digest ABJKNTUV on PSB1C3 and Promoter. (0.5h & 1h)=>Ligation =>transformation into TOP10 | Double Digest ABJKNTUV on PSB1C3 and Promoter. (0.5h & 1h)=>Ligation =>transformation into TOP10 | ||
| - | Construct pduAB under new standard.(condition tests:3h+PCR recycle+1h)=>ligation=>transformation into | + | Construct pduAB under new standard.(condition tests:3h+PCR recycle+1h)=>ligation=>transformation into TOP 10(on 9.12) |
| + | |||
| + | ---- | ||
| + | Hilde: | ||
| + | |||
| + | Ligation of pduABJK-pduNTUV | ||
| + | |||
| + | Fastdigest cutting of pduNTUV (X,P) & ABJK(S,P) | ||
| + | |||
| + | Ligation of pduABJK-pduNTUV | ||
Latest revision as of 09:15, 22 September 2010
Knightmare：
Plasmid extraction: ABJKNTUV on PSB1C3 in old standard with concentration and digestion determination.
Jocelyn:
Double Digest ABJKNTUV on PSB1C3 and Promoter. (0.5h & 1h)=>Ligation =>transformation into TOP10
Construct pduAB under new standard.(condition tests:3h+PCR recycle+1h)=>ligation=>transformation into TOP 10(on 9.12)
Hilde:
Ligation of pduABJK-pduNTUV
Fastdigest cutting of pduNTUV (X,P) & ABJK(S,P)
Ligation of pduABJK-pduNTUV
