Team:KAIST-Korea/Project/Methods/PCR Result

From 2010.igem.org

(Difference between revisions)
(→9/13 ~ 9/16 PCR Result)
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<table border=1>
<table border=1>
<tr align=center>
<tr align=center>
-
<td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">b</td><td width = "255px">STAT1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">c</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">c</td><td width = "255px">STAT1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">d</td><td width = "255px">STAT1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">e</td><td width = "255px">Ig-like</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">e</td><td width = "255px">STAT1</td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">f</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">f</td><td width = "255px">STAT1</td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">g</td><td width = "255px">Antibody</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">g</td><td width = "255px">V_STAT1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">h</td><td width = "255px">V_STAT1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">i</td><td width = "255px">V_STAT1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">j</td><td width = "255px">V_STAT1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
</tr>
</tr>
 +
<tr align=center>
 +
<td width = "40px">k</td><td width = "255px">V_STAT1</td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">l</td><td width = "255px">V_STAT1</td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
 +
</tr>
 +
</table>
</table>
<br>
<br>
<br>
<br>
-
[[Image:KAIST-exp1.png|560px]]
+
[[Image:KAIST-exp21.png|700px]]
<br>
<br>
<br>
<br>
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<table border=1>
<table border=1>
<tr align=center>
<tr align=center>
-
<td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">48*C</td><td width = "223px">60%</td><td width = "70px">2139bp</td>
+
<td width = "40px">a</td><td width = "255px">B_FGFR</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">48*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">b</td><td width = "255px">B_FGFR</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
</tr>
</tr>
 +
<tr align=center>
 +
<td width = "40px">c</td><td width = "255px">B_FGFR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">d</td><td width = "255px">B_FGFR</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">e</td><td width = "255px">B_FGFR</td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">f</td><td width = "255px">B_FGFR</td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">g</td><td width = "255px">VB_FGFR</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">h</td><td width = "255px">VB_FGFR</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">i</td><td width = "255px">VB_FGFR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">j</td><td width = "255px">VB_FGFR</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">k</td><td width = "255px">VB_FGFR</td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">l</td><td width = "255px">VB_FGFR</td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
 +
</tr>
 +
</table>
</table>
<br>
<br>
<br>
<br>
-
[[Image:KAIST-exp2.png|260px]]
+
[[Image:KAIST-exp22.png|700px]]
<br>
<br>
<br>
<br>
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<table border=1>
<table border=1>
<tr align=center>
<tr align=center>
-
<td width = "40px">a</td><td width = "255px"><font color = red>STAT1</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
+
<td width = "40px">a</td><td width = "255px">Signal peptide</td><td width = "200px">61*C</td><td width = "223px">30%</td><td width = "70px">87bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">b</td><td width = "255px">Ig-like</td><td width = "200px">61*C</td><td width = "223px">100%</td><td width = "70px">339bp</td>
</tr>
</tr>
-
</table>
 
-
<br>
 
-
<br>
 
-
[[Image:KAIST-exp3.png|260px]]
 
-
<br>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
<br>
 
-
<b>Exp 4</b>
 
-
<table border=1>
 
<tr align=center>
<tr align=center>
-
<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
+
<td width = "40px">c</td><td width = "255px">Signal peptide + Ig-like</td><td width = "200px">61*C</td><td width = "223px">100%</td><td width = "70px">426bp</td>
 +
</tr>
 +
<td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">59*C</td><td width = "223px">30%</td><td width = "70px">87bp</td>
</tr>
</tr>
-
</table>
 
-
<table border=1>
 
<tr align=center>
<tr align=center>
-
<td width = "40px">a</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">e</td><td width = "255px">Ig-like</td><td width = "200px">59*C</td><td width = "223px">100%</td><td width = "70px">339bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">b</td><td width = "255px">Signal peptide</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">f</td><td width = "255px">Signal peptide + Ig-like</td><td width = "200px">59*C</td><td width = "223px">100%</td><td width = "70px">426bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">c</td><td width = "255px">Ig-like</td><td width = "200px">50*C</td><td width = "223px">60%</td><td width = "70px">339bp</td>
+
<td width = "40px">g</td><td width = "255px">3'UTR</td><td width = "200px">52*C</td><td width = "223px">50%</td><td width = "70px">444bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">d</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">h</td><td width = "255px">3'UTR</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">444bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">e</td><td width = "255px">Antibody</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">i</td><td width = "255px">3'UTR</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
</tr>
</tr>
<tr align=center>
<tr align=center>
-
<td width = "40px">f</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
+
<td width = "40px">j</td><td width = "255px">3'UTR</td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">k</td><td width = "255px">3'UTR</td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">l</td><td width = "255px">5'UTR</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">m</td><td width = "255px">5'UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">n</td><td width = "255px">5'UTR</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">o</td><td width = "255px">5'UTR</td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 +
</tr>
 +
<tr align=center>
 +
<td width = "40px">p</td><td width = "255px">5'UTR</td><td width = "200px">69*C</td><td width = "223px">70%</td><td width = "70px">500bp</td>
</tr>
</tr>
</table>
</table>
<br>
<br>
<br>
<br>
-
[[Image:KAIST-exp4.png|300px]]
+
[[Image:KAIST-exp23.png|700px]]
-
<br>
+
-
<br>
+
<br>
<br>
<br>
<br>

Revision as of 19:32, 16 September 2010

 

PCR Experiment

9/6 ~ 9/9 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT153*C--
bV_STAT153*C--
cFusion Antibody receptor53*C--
dSignal peptide56*C--
eIg-like56*C--
fSignal Peptide + Ig-like56*C--
gAntibody56*C--
hV_Fusion Antibody Receptor53*C--
iB_FGFR156*C--
jVB_FGFR156*C--



KAIST-exp1.png




Exp 2

SegmentAnnealing TemperatureResult
aSTAT148*C60%2139bp
bV_STAT148*C--



KAIST-exp2.png




Exp 3

SegmentAnnealing TemperatureResult
aSTAT150*C100%2139bp
bV_STAT150*C--



KAIST-exp3.png




Exp 4

SegmentAnnealing TemperatureResult
aFusion Antibody receptor50*C--
bSignal peptide50*C--
cIg-like50*C60%339bp
dSignal Peptide + Ig-like50*C--
eAntibody50*C--
fV_Fusion Antibody Receptor50*C--



KAIST-exp4.png






9/10 ~ 9/12 PCR Result

PCR composition

MaterialsAmount
Template150pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)5ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT160*C--
bV_STAT160*C--
cFusion Antibody receptor60*C70%1095bp
dSignal peptide60*C--
eIg-like60*C60%339bp
fSignal Peptide + Ig-like60*C--
gAntibody60*C100%669bp
hV_Fusion Antibody Receptor60*C--
iB_FGFR160*C--
jVB_FGFR160*C--
x1negative 160*C--
x2negative 260*C--



KAIST-exp5.png





Exp 2

SegmentAnnealing TemperatureResult
aSTAT158*C60%2139bp
bV_STAT158*C--
cFusion Antibody receptor58*C80%1095bp
dSignal peptide58*C80%87bp
eIg-like58*C100%339bp
fSignal Peptide + Ig-like58*C80%426bp
gAntibody58*C100%669bp
hV_Fusion Antibody Receptor58*C--
iB_FGFR158*C--
jVB_FGFR158*C--
xnegative58*C--



KAIST-exp6.png




Exp 3

SegmentAnnealing TemperatureResult
aSTAT162*C--
bV_STAT162*C--
cFusion Antibody receptor62*C10%1095bp
dSignal peptide62*C--
eIg-like62*C--
fSignal Peptide + Ig-like62*C--
gAntibody62*C--
hV_Fusion Antibody Receptor62*C--
iB_FGFR162*C--
jVB_FGFR162*C--
xnegative62*C--



KAIST-exp7.png




Exp 4

SegmentAnnealing TemperatureResult
aSTAT156*C--
bV_STAT156*C--
cFusion Antibody receptor56*C5%1095bp
dSignal peptide56*C--
eIg-like56*C80%339bp
fSignal Peptide + Ig-like56*C--
gAntibody56*C80%669bp
hV_Fusion Antibody Receptor56*C--
iB_FGFR156*C--
jVB_FGFR156*C--
xnegative56*C--



KAIST-exp8.png




Exp 5

SegmentAnnealing TemperatureResult
aV_STAT164*C--
bB_FGFR164*C5%1398bp
cVB_FGFR164*C--



KAIST-exp9.png





Exp 6

SegmentAnnealing TemperatureResult
aV_STAT164*C--
bB_FGFR164*C3%1398bp
cVB_FGFR164*C--



KAIST-exp10.png





Exp 7

SegmentAnnealing TemperatureResult
a3'UTR62*C--
b5'UTR62*C--









Exp 8

SegmentAnnealing TemperatureResult
aV_STAT168*C--
bB_FGFR168*C10%1398bp
cVB_FGFR168*C--



KAIST-exp12.png





Exp 9

SegmentAnnealing TemperatureResult
aV_STAT170*C--
bB_FGFR170*C3%1398bp
cVB_FGFR170*C--
c5'UTR70*C--
c3'UTR70*C--



KAIST-exp13.png





Exp 10

SegmentAnnealing TemperatureResult
aV_STAT167*C--
bB_FGFR167*C6%1398bp
cVB_FGFR167*C--



KAIST-exp14.png





9/13 ~ 9/16 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT150*C100%2139bp
bSTAT152*C100%2139bp
cSTAT154*C100%2139bp
dSTAT156*C100%2139bp
eSTAT158*C100%2139bp
fSTAT160*C100%2139bp
gV_STAT150*C100%2151bp
hV_STAT152*C100%2151bp
iV_STAT154*C100%2151bp
jV_STAT156*C100%2151bp
kV_STAT158*C100%2151bp
lV_STAT160*C100%2151bp



KAIST-exp21.png




Exp 2

SegmentAnnealing TemperatureResult
aB_FGFR50*C100%1398bp
bB_FGFR52*C100%1398bp
cB_FGFR54*C100%1398bp
dB_FGFR56*C100%1398bp
eB_FGFR58*C100%1398bp
fB_FGFR60*C100%1398bp
gVB_FGFR50*C100%1420bp
hVB_FGFR52*C100%1420bp
iVB_FGFR54*C100%1420bp
jVB_FGFR56*C100%1420bp
kVB_FGFR58*C100%1420bp
lVB_FGFR60*C100%1420bp



KAIST-exp22.png




Exp 3

SegmentAnnealing TemperatureResult

</tr>

aSignal peptide61*C30%87bp
bIg-like61*C100%339bp
cSignal peptide + Ig-like61*C100%426bp
dSignal peptide59*C30%87bp
eIg-like59*C100%339bp
fSignal peptide + Ig-like59*C100%426bp
g3'UTR52*C50%444bp
h3'UTR54*C50%444bp
i3'UTR56*C100%444bp
j3'UTR58*C100%444bp
k3'UTR60*C100%444bp
l5'UTR52*C100%500bp
m5'UTR54*C100%500bp
n5'UTR56*C100%500bp
o5'UTR58*C100%500bp
p5'UTR69*C70%500bp



KAIST-exp23.png




Retrieved from "http://2010.igem.org/Team:KAIST-Korea/Project/Methods/PCR_Result"