Team:ETHZ Basel/InformationProcessing/Visualization

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The cells were placed in a 50 μm (?) high flow chamber ([https://2010.igem.org/Team:ETHZ_Basel/InformationProcessing/MicroscopeSetup Details]).  
The cells were placed in a 50 μm (?) high flow chamber ([https://2010.igem.org/Team:ETHZ_Basel/InformationProcessing/MicroscopeSetup Details]).  
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The movies were made out of bright field images. The excitation was 60ms, the period something around 1/7s, pretty much the fps rate of the movie (we can go heigher, but yet we already produced like 2 GB of raw images for every movie). Every image has the size of 1344 x 1024 pixels, 12 or 16 bit grayscale (I forgot what I have chosen, probably we will reduce it anyway later to speed up the imaging pipeline). For both movies the images were made out-of-focus to simplify cell detection.
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The excitation for the bright field images (40x) was 60ms, the period something around 0.3s, aproximately corresponding to the fps rate of the movie. To increase speed, the binning in the microscope was set to 2, so that the images were 672 x 512 grayscale (16 bit) instead of the maximal resolution of the microscope of 1344 x 1024 pixels, and every pixel  The images were made out-of-focus to simplify cell detection.

Revision as of 12:19, 16 September 2010

Visualization

The cells were placed in a 50 μm (?) high flow chamber (Details). The excitation for the bright field images (40x) was 60ms, the period something around 0.3s, aproximately corresponding to the fps rate of the movie. To increase speed, the binning in the microscope was set to 2, so that the images were 672 x 512 grayscale (16 bit) instead of the maximal resolution of the microscope of 1344 x 1024 pixels, and every pixel The images were made out-of-focus to simplify cell detection.

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