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Team:Cambridge/Notebook/10
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{{Team:Cambridge/Templates/Day|Day=Monday}} | {{Team:Cambridge/Templates/Day|Day=Monday}} | ||
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| - | To our pleasant surprise our plates of P. pyralis in pSB1C3, L. cruciata in pSB1C3 and L. cruciata with pBAD in SB1C3 had all grown successfully over the weekend. Only P. pyralis with pBAD had not grown, which Hannah and Emily (who was back from holiday) repeated that experiment today. | + | To our pleasant surprise our plates of P. pyralis in pSB1C3, L. cruciata in pSB1C3 and L. cruciata with pBAD in SB1C3 had all grown successfully over the weekend. Only P. pyralis with pBAD had not grown, which Hannah and Emily (who was back from holiday) repeated that experiment today. |
| - | In trying to be organised, we've also grown up broths of the original DNA2.0 plasmids and the products of the plates to make glycerol stocks tomorrow. | + | In the meantime, Bill investigated flights to America and Theo wrote a draft abstract which the team discussed over coffee. The thioesterase plates had also grown over the weekend as well, which was very exciting! Ben set up a plate reader experiment going overnight to test whether L. cruciata luciferase worked and Theo took very pretty pictures of light bulbs glowing with bacteria! |
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| + | In trying to be organised, we've also grown up broths of the original DNA2.0 plasmids and the products of the plates to make glycerol stocks tomorrow. Ben put on a plate reader experiment in the evening while Theo made overnight cultures that could be sent for sequencing. | ||
{{Team:Cambridge/Templates/Day|Day=Tuesday}} | {{Team:Cambridge/Templates/Day|Day=Tuesday}} | ||
Revision as of 16:43, 14 September 2010
Contents |
Monday
To our pleasant surprise our plates of P. pyralis in pSB1C3, L. cruciata in pSB1C3 and L. cruciata with pBAD in SB1C3 had all grown successfully over the weekend. Only P. pyralis with pBAD had not grown, which Hannah and Emily (who was back from holiday) repeated that experiment today.
In the meantime, Bill investigated flights to America and Theo wrote a draft abstract which the team discussed over coffee. The thioesterase plates had also grown over the weekend as well, which was very exciting! Ben set up a plate reader experiment going overnight to test whether L. cruciata luciferase worked and Theo took very pretty pictures of light bulbs glowing with bacteria!
In trying to be organised, we've also grown up broths of the original DNA2.0 plasmids and the products of the plates to make glycerol stocks tomorrow. Ben put on a plate reader experiment in the evening while Theo made overnight cultures that could be sent for sequencing.
Tuesday
Wednesday
luorescent proteins onto the two operons, continuing Tuedays experiment and plated these out.
Thursday
Friday
Saturday
Sunday
