Team:UNIPV-Pavia/Calendar/July

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Revision as of 20:36, 13 September 2010

JULY

Bio-Lab activity proceeds!

Select the day you are interested in to find out the details!

DATE	ACTIVITY
July, 1st	19° Bio-Lab - I7-3,4 and 5 and I8-3, 4 and 5 were prepared for TECAN test. Of the plates incubated yesterday, a colony was picked and grown in 1ml LB (+ antibiotic) for 6 hours at 37°C 220 rpm. Other parts were received from Anderson Lab.
July, 2nd	20° Bio-Lab - TECAN test provided encouraging results, showing that I7-3, I7-5, I8-4 and I8-5 produced GFP. Other culture (I7-1, I7-2, I7-4, I8-1, I8-2, I8-3) did not produce GFP, so they were thrown away. A furhter screening were performed on I7-3, I7-5, I8-4, I8-5 and this time all the clones are OK . Inoculum of <partinfo>BBa_J72007</partinfo> and <partinfo>BBa_J72013</partinfo> from LB agar plates.A glycerol stock was prepared for each culture. Inoculum of <partinfo>BBa_J72008</partinfo> from LB agar plate grown at 30°C 220 rpm overnight.
JULY: WEEK 2
July, 5th	21° Bio-Lab - LB agar plates prepared.
July, 6th	22° Bio-Lab - We decided to perform a TECAN test to evaluate the strength of some promoters belonging to the Anderson Promoters Collection.
July, 7th	23° Bio-Lab -Competent cells preparation for: MG1655 BW53474 BW25141 BW25142 PCR was performed to amplify Phasins. 11° Meeting - Updating about Bio-Lab activity.
July, 8th	24° Bio-Lab - Transformation of <partinfo>BBa_J23118</partinfo> in our home-made competent strains MG1655 BW25141 BW25142 BW53474 DH5alpha (as control) to check transformation efficiency.
July, 9th	25° Bio-Lab - All plates showed red colonies, so we calculated the efficiency of the strains.
JULY: WEEK 3
July, 12th	26° Bio-Lab - Phasins PhaP1 and PhaP2 were amplified by PCR and the results were sequenced .
July, 13th	27° Bio-Lab - Phasins samples were sent to be sequenced. MiniPrep was performed. Ligations (from I11 till I19 and I74c5, I84c5, I104c5) were all performed
July, 14th	28° Bio-Lab - PBHR68 plate showed colonies!!transformation of ligation of yesterday. Screening of <partinfo>BBa_J72007</partinfo> (CRIM), <partinfo>BBa_J72008</partinfo> (helper), <partinfo>pAH123</partinfo> (helper) ans <partinfo>pCP20</partinfo> (helper).
July, 15th	29° Bio-Lab -agar plates after transformation were checked(2 colonies were picked from every plate). MiniPrep was performed for: PBHR68 (BioPlastic operon) -> 272 ng/ul BBa_J72007(CRIM plasmid) -> 41 ng/ul (not clean spectrum at 230nm) BBa_J72013(CRIM plasmid) -> 16 ng/ul
July, 16th	30° Bio-Lab - MiniPrep was performed on 23 falcon tubes containing I11 -> I19 and I7/8/10-4C5 ligations. We decided to performe a TECAN test on our parts, in order to see if RFP was correctly excided from BBa_J231xx vector, since length of RFP and of our insert (RBS-luxI-tt) were similar.
JULY: WEEK 4
July, 19th	31° Bio-Lab -
July, 20th	32° Bio-Lab -
July, 21st	33° Bio-Lab -
July, 22nd	34° Bio-Lab -
July, 23rd	35° Bio-Lab -
July, 24th	36° Bio-Lab -
July, 25th	37° Bio-Lab -
JULY: WEEK 5
July, 26th	38° Bio-Lab - 12° Meeting - Updating about Bio-Lab activity and organization of our trip to Boston!
July, 27th	39° Bio-Lab -
July, 28th	40° Bio-Lab -
July, 29th	41° Bio-Lab -
July, 30th	42° Bio-Lab -
July, 31th	43° Bio-Lab -

@@ Line 78: / Line 78: @@
 <tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 3</b></th></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 12th|July, 12th]]</td><td align="left">
-° Bio-Lab -
+° Bio-Lab - Phasins PhaP1 and PhaP2 were amplified by PCR and the results were sequenced .
 </td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 13th|July, 13th]]</td><td align="left">
-° Bio-Lab -
+° Bio-Lab - Phasins samples were sent to be sequenced. MiniPrep was performed. Ligations (from I11 till I19 and I74c5, I84c5, I104c5) were all performed
 </td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 14th|July, 14th]]</td><td align="left">
-° Bio-Lab -
+° Bio-Lab - PBHR68 plate showed colonies!!transformation of ligation of yesterday. Screening of <partinfo>BBa_J72007</partinfo> (CRIM), <partinfo>BBa_J72008</partinfo> (helper), <partinfo>pAH123</partinfo> (helper) ans <partinfo>pCP20</partinfo> (helper).
 </td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 15th|July, 15th]]</td><td align="left">
-° Bio-Lab -
+° Bio-Lab -agar plates after transformation were checked(2 colonies were picked from every plate).
+MiniPrep was performed for:
+* PBHR68 (BioPlastic operon) -> 272 ng/ul
+* BBa_J72007(CRIM plasmid) -> 41 ng/ul (not clean spectrum at 230nm)
+* BBa_J72013(CRIM plasmid) -> 16 ng/ul
 </td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana3 #July, 16th|July, 16th]]</td><td align="left">
-° Bio-Lab -
+° Bio-Lab - MiniPrep was performed on 23 falcon tubes containing I11 -> I19 and I7/8/10-4C5 ligations.
+We decided to performe a TECAN test  on our parts, in order to see if RFP was correctly excided from BBa_J231xx vector, since length of RFP and of our insert (RBS-luxI-tt) were similar.
 </td></tr>
 <tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 4</b></th></tr>