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- | <span style=font-size:30px><font face="Maiandra GD"><b>PCR Result</b></font></span> | + | <span style=font-size:30px><font face="Maiandra GD"><b>PCR Experiment</b></font></span> |
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Revision as of 17:14, 12 September 2010
PCR Experiment
9/6 ~ 9/9 PCR Result
PCR composition
Materials | Amount |
Template | 100pmole |
Primer A | 50pmole |
Primer B | 50pmole |
dNTP (dNTP mixture 2.5mM TaKaRa) | 2ul |
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE) | 0.25ul |
10x buffer (10x cloned Pfu Reaction buffer) | 5ul |
DW (3rd sterile water) | rest |
Total | 50ul |
Exp 1
Segment | Annealing Temperature | Result |
a | STAT1 | 53*C | - | - |
b | V_STAT1 | 53*C | - | - |
c | Fusion Antibody receptor | 53*C | - | - |
d | Signal peptide | 56*C | - | - |
e | Ig-like | 56*C | - | - |
f | Signal Peptide + Ig-like | 56*C | - | - |
g | Antibody | 56*C | - | - |
h | V_Fusion Antibody Receptor | 53*C | - | - |
i | B_FGFR1 | 56*C | - | - |
j | VB_FGFR1 | 56*C | - | - |
Exp 2
Segment | Annealing Temperature | Result |
a | STAT1 | 48*C | 60% | 2139bp |
b | V_STAT1 | 48*C | - | - |
Exp 3
Segment | Annealing Temperature | Result |
a | STAT1 | 50*C | 100% | 2139bp |
b | V_STAT1 | 50*C | - | - |
Exp 4
Segment | Annealing Temperature | Result |
a | Fusion Antibody receptor | 50*C | - | - |
b | Signal peptide | 50*C | - | - |
c | Ig-like | 50*C | 60% | 339bp |
d | Signal Peptide + Ig-like | 50*C | - | - |
e | Antibody | 50*C | - | - |
f | V_Fusion Antibody Receptor | 50*C | - | - |
9/10 ~ 9/12 PCR Result
PCR composition
Materials | Amount |
Template | 150pmole |
Primer A | 50pmole |
Primer B | 50pmole |
dNTP (dNTP mixture 2.5mM TaKaRa) | 5ul |
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE) | 0.25ul |
10x buffer (10x cloned Pfu Reaction buffer) | 5ul |
DW (3rd sterile water) | rest |
Total | 50ul |
Exp 1
Segment | Annealing Temperature | Result |
a | STAT1 | 60*C | - | - |
b | V_STAT1 | 60*C | - | - |
c | Fusion Antibody receptor | 60*C | 70% | 1095bp |
d | Signal peptide | 60*C | - | - |
e | Ig-like | 60*C | 60% | 339bp |
f | Signal Peptide + Ig-like | 60*C | - | - |
g | Antibody | 60*C | 100% | 669bp |
h | V_Fusion Antibody Receptor | 60*C | - | - |
i | B_FGFR1 | 60*C | - | - |
j | VB_FGFR1 | 60*C | - | - |
x1 | negative 1 | 60*C | - | - |
x2 | negative 2 | 60*C | - | - |
Exp 2
Segment | Annealing Temperature | Result |
a | STAT1 | 58*C | 60% | 2139bp |
b | V_STAT1 | 58*C | - | - |
c | Fusion Antibody receptor | 58*C | 80% | 1095bp |
d | Signal peptide | 58*C | 80% | 87bp |
e | Ig-like | 58*C | 100% | 339bp |
f | Signal Peptide + Ig-like | 58*C | 80% | 426bp |
g | Antibody | 58*C | 100% | 669bp |
h | V_Fusion Antibody Receptor | 58*C | - | - |
i | B_FGFR1 | 58*C | - | - |
j | VB_FGFR1 | 58*C | - | - |
x | negative | 58*C | - | - |
Exp 3
Segment | Annealing Temperature | Result |
a | STAT1 | 62*C | - | - |
b | V_STAT1 | 62*C | - | - |
c | Fusion Antibody receptor | 62*C | 10% | 1095bp |
d | Signal peptide | 62*C | - | - |
e | Ig-like | 62*C | - | - |
f | Signal Peptide + Ig-like | 62*C | - | - |
g | Antibody | 62*C | - | - |
h | V_Fusion Antibody Receptor | 62*C | - | - |
i | B_FGFR1 | 62*C | - | - |
j | VB_FGFR1 | 62*C | - | - |
x | negative | 62*C | - | - |
Exp 4
Segment | Annealing Temperature | Result |
a | STAT1 | 56*C | - | - |
b | V_STAT1 | 56*C | - | - |
c | Fusion Antibody receptor | 56*C | 5% | 1095bp |
d | Signal peptide | 56*C | - | - |
e | Ig-like | 56*C | 80% | 339bp |
f | Signal Peptide + Ig-like | 56*C | - | - |
g | Antibody | 56*C | 80% | 669bp |
h | V_Fusion Antibody Receptor | 56*C | - | - |
i | B_FGFR1 | 56*C | - | - |
j | VB_FGFR1 | 56*C | - | - |
x | negative | 56*C | - | - |
Exp 5
Segment | Annealing Temperature | Result |
a | V_STAT1 | 64*C | - | - |
b | B_FGFR1 | 64*C | 5% | 1398bp |
c | VB_FGFR1 | 64*C | - | - |
Exp 6
Segment | Annealing Temperature | Result |
a | V_STAT1 | 64*C | - | - |
b | B_FGFR1 | 64*C | 3% | 1398bp |
c | VB_FGFR1 | 64*C | - | - |
Exp 7
Segment | Annealing Temperature | Result |
a | 3'UTR | 62*C | - | - |
b | 5'UTR | 62*C | - | - |
Exp 8
Segment | Annealing Temperature | Result |
a | V_STAT1 | 68*C | - | - |
b | B_FGFR1 | 68*C | 10% | 1398bp |
c | VB_FGFR1 | 68*C | - | - |
Exp 9
Segment | Annealing Temperature | Result |
a | V_STAT1 | 70*C | - | - |
b | B_FGFR1 | 70*C | 3% | 1398bp |
c | VB_FGFR1 | 70*C | - | - |
c | 5'UTR | 70*C | - | - |
c | 3'UTR | 70*C | - | - |
Exp 10
Segment | Annealing Temperature | Result |
a | V_STAT1 | 67*C | - | - |
b | B_FGFR1 | 67*C | 6% | 1398bp |
c | VB_FGFR1 | 67*C | - | - |
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