Team:KAIST-Korea/Project/Methods/PCR Result

From 2010.igem.org

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(→9/10 ~ 9/12 PCR Result)
(→9/10 ~ 9/12 PCR Result)
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<td width = "40px">x1</td><td width = "255px">negative</td><td width = "200px">58*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">x</td><td width = "255px">negative</td><td width = "200px">58*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">c</td><td width = "255px"><font color = blue>Fusion Antibody receptor</font></td><td width = "200px">62*C</td><td width = "223px">10%</td><td width = "70px">1095bp</td>
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<td width = "40px">c</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">62*C</td><td width = "223px">10%</td><td width = "70px">1095bp</td>
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<td width = "40px">d</td><td width = "255px"><font color = blue>Signal peptide</font></td><td width = "200px">58*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">f</td><td width = "255px"><font color = blue>Signal Peptide + Ig-like</font></td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">f</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">g</td><td width = "255px"><font color = red>Antibody</font></td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">g</td><td width = "255px">Antibody</font><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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<tr align=center>
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<td width = "40px">x1</td><td width = "255px">negative</td><td width = "200px">58*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">x</td><td width = "255px">negative</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
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[[Image:KAIST-exp7.png|640px]]
[[Image:KAIST-exp7.png|640px]]
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<b>Exp 4</b>
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<table border=1>
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<tr align=center>
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<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
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<td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">c</td><td width = "255px"><font color = blue>Fusion Antibody receptor</font></td><td width = "200px">56*C</td><td width = "223px">5%</td><td width = "70px">1095bp</td>
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<td width = "40px">d</td><td width = "255px"><font color = blue>Signal peptide</font></td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">e</td><td width = "255px"><font color = red>Ig-like</font></td><td width = "200px">56*C</td><td width = "223px">80</td><td width = "70px">339bp</td>
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<td width = "40px">f</td><td width = "255px"><font color = blue>Signal Peptide + Ig-like</font></td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">g</td><td width = "255px"><font color = red>Antibody</font></td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">x</td><td width = "255px">negative</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
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[[Image:KAIST-exp7.png|640px]]
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Revision as of 16:17, 12 September 2010

 

PCR Result

9/6 ~ 9/9 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT153*C--
bV_STAT153*C--
cFusion Antibody receptor53*C--
dSignal peptide56*C--
eIg-like56*C--
fSignal Peptide + Ig-like56*C--
gAntibody56*C--
hV_Fusion Antibody Receptor53*C--
iB_FGFR156*C--
jVB_FGFR156*C--



KAIST-exp1.png




Exp 2

SegmentAnnealing TemperatureResult
aSTAT148*C60%2139bp
bV_STAT148*C--



KAIST-exp2.png




Exp 3

SegmentAnnealing TemperatureResult
aSTAT150*C100%2139bp
bV_STAT150*C--



KAIST-exp3.png




Exp 4

SegmentAnnealing TemperatureResult
aFusion Antibody receptor50*C--
bSignal peptide50*C--
cIg-like50*C60%339bp
dSignal Peptide + Ig-like50*C--
eAntibody50*C--
fV_Fusion Antibody Receptor50*C--



KAIST-exp4.png






9/10 ~ 9/12 PCR Result

PCR composition

MaterialsAmount
Template150pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)5ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT160*C--
bV_STAT160*C--
cFusion Antibody receptor60*C70%1095bp
dSignal peptide60*C--
eIg-like60*C60%339bp
fSignal Peptide + Ig-like60*C--
gAntibody60*C100%669bp
hV_Fusion Antibody Receptor60*C--
iB_FGFR160*C--
jVB_FGFR160*C--
x1negative 160*C--
x2negative 260*C--



KAIST-exp5.png





Exp 2

SegmentAnnealing TemperatureResult
aSTAT158*C60%2139bp
bV_STAT158*C--
cFusion Antibody receptor58*C80%1095bp
dSignal peptide58*C80%87bp
eIg-like58*C100%339bp
fSignal Peptide + Ig-like58*C80%426bp
gAntibody58*C100%669bp
hV_Fusion Antibody Receptor58*C--
iB_FGFR158*C--
jVB_FGFR158*C--
xnegative58*C--



KAIST-exp6.png




Exp 3

SegmentAnnealing TemperatureResult
<td width = "223px">-</td><td width = "70px">-</td> <tr align=center> <td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td> </tr> <tr align=center> <td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td> </tr> <tr align=center> <td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td> </tr> <tr align=center> <td width = "40px">x</td><td width = "255px">negative</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td> </tr> </table>

KAIST-exp7.png




Exp 4
aSTAT162*C--
bV_STAT162*C--
cFusion Antibody receptor62*C10%1095bp
dSignal peptide62*C--
eIg-like62*C--
fSignal Peptide + Ig-like62*C--
gAntibody</font><td width = "200px">62*C
SegmentAnnealing TemperatureResult
aSTAT156*C--
bV_STAT156*C--
cFusion Antibody receptor56*C5%1095bp
dSignal peptide56*C--
eIg-like56*C80339bp
fSignal Peptide + Ig-like56*C--
gAntibody56*C--
hV_Fusion Antibody Receptor56*C--
iB_FGFR156*C--
jVB_FGFR156*C--
xnegative56*C--



KAIST-exp7.png




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