From 2010.igem.org
(Difference between revisions)
|
|
| Line 308: |
Line 308: |
| | <br> | | <br> |
| | <br> | | <br> |
| - | <b>Exp 2</b> | + | <b>Exp 3</b> |
| | <table border=1> | | <table border=1> |
| | <tr align=center> | | <tr align=center> |
Revision as of 16:11, 12 September 2010
|
PCR Result
9/6 ~ 9/9 PCR Result
PCR composition
| Materials | Amount |
| Template | 100pmole |
| Primer A | 50pmole |
| Primer B | 50pmole |
| dNTP (dNTP mixture 2.5mM TaKaRa) | 2ul |
| Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE) | 0.25ul |
| 10x buffer (10x cloned Pfu Reaction buffer) | 5ul |
| DW (3rd sterile water) | rest |
| Total | 50ul |
Exp 1
| Segment | Annealing Temperature | Result |
| a | STAT1 | 53*C | - | - |
| b | V_STAT1 | 53*C | - | - |
| c | Fusion Antibody receptor | 53*C | - | - |
| d | Signal peptide | 56*C | - | - |
| e | Ig-like | 56*C | - | - |
| f | Signal Peptide + Ig-like | 56*C | - | - |
| g | Antibody | 56*C | - | - |
| h | V_Fusion Antibody Receptor | 53*C | - | - |
| i | B_FGFR1 | 56*C | - | - |
| j | VB_FGFR1 | 56*C | - | - |
Exp 2
| Segment | Annealing Temperature | Result |
| a | STAT1 | 48*C | 60% | 2139bp |
| b | V_STAT1 | 48*C | - | - |
Exp 3
| Segment | Annealing Temperature | Result |
| a | STAT1 | 50*C | 100% | 2139bp |
| b | V_STAT1 | 50*C | - | - |
Exp 4
| Segment | Annealing Temperature | Result |
| a | Fusion Antibody receptor | 50*C | - | - |
| b | Signal peptide | 50*C | - | - |
| c | Ig-like | 50*C | 60% | 339bp |
| d | Signal Peptide + Ig-like | 50*C | - | - |
| e | Antibody | 50*C | - | - |
| f | V_Fusion Antibody Receptor | 50*C | - | - |
9/10 ~ 9/12 PCR Result
PCR composition
| Materials | Amount |
| Template | 150pmole |
| Primer A | 50pmole |
| Primer B | 50pmole |
| dNTP (dNTP mixture 2.5mM TaKaRa) | 5ul |
| Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE) | 0.25ul |
| 10x buffer (10x cloned Pfu Reaction buffer) | 5ul |
| DW (3rd sterile water) | rest |
| Total | 50ul |
Exp 1
| Segment | Annealing Temperature | Result |
| a | STAT1 | 60*C | - | - |
| b | V_STAT1 | 60*C | - | - |
| c | Fusion Antibody receptor | 60*C | 70% | 1095bp |
| d | Signal peptide | 60*C | - | - |
| e | Ig-like | 60*C | 60% | 339bp |
| f | Signal Peptide + Ig-like | 60*C | - | - |
| g | Antibody | 60*C | 100% | 669bp |
| h | V_Fusion Antibody Receptor | 60*C | - | - |
| i | B_FGFR1 | 60*C | - | - |
| j | VB_FGFR1 | 60*C | - | - |
| x1 | negative 1 | 60*C | - | - |
| x2 | negative 2 | 60*C | - | - |
Exp 2
| Segment | Annealing Temperature | Result |
| a | STAT1 | 58*C | 60% | 2139bp |
| b | V_STAT1 | 58*C | - | - |
| c | Fusion Antibody receptor | 58*C | 80% | 1095bp |
| d | Signal peptide | 58*C | 80% | 87bp |
| e | Ig-like | 58*C | 100% | 339bp |
| f | Signal Peptide + Ig-like | 58*C | 80% | 426bp |
| g | Antibody | 58*C | 100% | 669bp |
| h | V_Fusion Antibody Receptor | 58*C | - | - |
| i | B_FGFR1 | 58*C | - | - |
| j | VB_FGFR1 | 58*C | - | - |
| x1 | negative | 58*C | - | - |
Exp 3
| Segment | Annealing Temperature | Result |
| a | STAT1 | 62*C | - | - |
| b | V_STAT1 | 62*C | - | - |
| c | Fusion Antibody receptor | 62*C | 10% | 1095bp |
| d | Signal peptide | 58*C | - | - |
| e | Ig-like | 62*C | - | - |
| f | Signal Peptide + Ig-like | 62*C | - | - |
| g | Antibody | 62*C | - | - |
| h | V_Fusion Antibody Receptor | 62*C | - | - |
| i | B_FGFR1 | 62*C | - | - |
| j | VB_FGFR1 | 62*C | - | - |
| x1 | negative | 58*C | - | - |
 |
"
