Team:KAIST-Korea/Project/Methods/PCR Result

From 2010.igem.org

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(→9/10 ~ 9/12 PCR Result)
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==9/10 ~ 9/12 PCR Result==
==9/10 ~ 9/12 PCR Result==
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<span style=font-size:12px><b>PCR composition </b></span>
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<table border=1>
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<tr align=center>
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<td><b>Materials</b></td><td><b>Amount</b></td>
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</tr>
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<tr align=center>
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<td>Template</td><td>150pmole</td>
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</tr>
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<tr align=center>
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<td>Primer A</td><td>50pmole</td>
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</tr>
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<tr align=center>
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<td>Primer B</td><td>50pmole</td>
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</tr>
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<tr align=center>
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<td>dNTP (dNTP mixture 2.5mM TaKaRa)</td><td>5ul</td>
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</tr>
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<tr align=center>
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<td>Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)</td><td>0.25ul</td>
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</tr>
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<tr align=center>
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<td>10x buffer (10x cloned Pfu Reaction buffer)</td><td>5ul</td>
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</tr>
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<tr align=center>
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<td>DW (3rd sterile water)</td><td>rest</td>
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</tr>
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<tr align=center>
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<td>Total</td><td>50ul</td>
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</tr>
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</table>
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<br>
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<br>
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<b>Exp 1</b>
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<table border=1>
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<tr align=center>
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<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
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</tr>
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</table>
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<table border=1>
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<tr align=center>
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<td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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<tr align=center>
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<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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<tr align=center>
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<td width = "40px">c</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">60*C</td><td width = "223px">70%</td><td width = "70px">1095bp</td>
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</tr>
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<tr align=center>
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<td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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<tr align=center>
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<td width = "40px">e</td><td width = "255px">Ig-like</td><td width = "200px">60*C</td><td width = "223px">60%</td><td width = "70px">339bp</td>
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</tr>
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<tr align=center>
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<td width = "40px">f</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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<tr align=center>
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<td width = "40px">g</td><td width = "255px"><font color = red>Antibody</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">669bp</td>
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</tr>
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<tr align=center>
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<td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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<tr align=center>
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<td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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<tr align=center>
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<td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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<tr align=center>
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<td width = "40px">x1</td><td width = "255px">negative 1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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<tr align=center>
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<td width = "40px">x2</td><td width = "255px">negative 2</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
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</tr>
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</table>
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<br>
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<br>
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[[Image:KAIST-exp1.png|560px]]
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<br>
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<br>

Revision as of 15:55, 12 September 2010

 

PCR Result

9/6 ~ 9/9 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT153*C--
bV_STAT153*C--
cFusion Antibody receptor53*C--
dSignal peptide56*C--
eIg-like56*C--
fSignal Peptide + Ig-like56*C--
gAntibody56*C--
hV_Fusion Antibody Receptor53*C--
iB_FGFR156*C--
jVB_FGFR156*C--



KAIST-exp1.png

Exp 2

SegmentAnnealing TemperatureResult
aSTAT148*C60%2139bp
bV_STAT148*C--



KAIST-exp2.png

Exp 3

SegmentAnnealing TemperatureResult
aSTAT150*C100%2139bp
bV_STAT150*C--



KAIST-exp3.png

Exp 4

SegmentAnnealing TemperatureResult
aFusion Antibody receptor50*C--
bSignal peptide50*C--
cIg-like50*C60%339bp
dSignal Peptide + Ig-like50*C--
eAntibody50*C--
fV_Fusion Antibody Receptor50*C--



KAIST-exp4.png


9/10 ~ 9/12 PCR Result

PCR composition

MaterialsAmount
Template150pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)5ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT160*C--
bV_STAT160*C--
cFusion Antibody receptor60*C70%1095bp
dSignal peptide60*C--
eIg-like60*C60%339bp
fSignal Peptide + Ig-like60*C--
gAntibody60*C100%669bp
hV_Fusion Antibody Receptor60*C--
iB_FGFR160*C--
jVB_FGFR160*C--
x1negative 160*C--
x2negative 260*C--



KAIST-exp1.png


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