From 2010.igem.org
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Revision as of 15:50, 12 September 2010
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PCR Result
9/6 ~ 9/9 PCR Result
PCR composition
| Materials | Amount |
| Template | 100pmole |
| Primer A | 50pmole |
| Primer B | 50pmole |
| dNTP (dNTP mixture 2.5mM TaKaRa) | 2ul |
| Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE) | 0.25ul |
| 10x buffer (10x cloned Pfu Reaction buffer) | 5ul |
| DW (3rd sterile water) | rest |
| Total | 50ul |
Exp 1
| Segment | Annealing Temperature | Result |
| a | STAT1 | 53*C | - | - |
| b | V_STAT1 | 53*C | - | - |
| c | Fusion Antibody receptor | 53*C | - | - |
| d | Signal peptide | 56*C | - | - |
| e | Ig-like | 56*C | - | - |
| f | Signal Peptide + Ig-like | 56*C | - | - |
| g | Antibody | 56*C | - | - |
| h | V_Fusion Antibody Receptor | 53*C | - | - |
| i | B_FGFR1 | 56*C | - | - |
| j | VB_FGFR1 | 56*C | - | - |
Exp 2
| Segment | Annealing Temperature | Result |
| a | STAT1 | 48*C | 60% | 2139bp |
| b | V_STAT1 | 48*C | - | - |
Exp 3
| Segment | Annealing Temperature | Result |
| a | STAT1 | 50*C | 100% | 2139bp |
| b | V_STAT1 | 50*C | - | - |
Exp 4
| Segment | Annealing Temperature | Result |
| a | Fusion Antibody receptor | 50*C | - | - |
| b | Signal peptide | 50*C | - | - |
| c | Ig-like | 50*C | 60% | 339bp |
| d | Signal Peptide + Ig-like | 50*C | - | - |
| e | Antibody | 50*C | - | - |
| f | V_Fusion Antibody Receptor | 50*C | - | - |
9/10 ~ 9/12 PCR Result
dd |
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