Team:KAIST-Korea/Project/Methods/PCR Result

From 2010.igem.org

(Difference between revisions)
(→9/6 ~ 9/9 PCR Result)
(→9/6 ~ 9/9 PCR Result)
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<b>exp 1</b>
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<b>Exp 1</b>
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<b>exp 2</b>
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<b>Exp 2</b>
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<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
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<td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">48*C</td><td width = "223px">60%</td><td width = "70px">2139bp</td>
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<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">48*C</td><td width = "223px">-</td><td width = "70px">-</td>
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[[Image:KAIST-exp2.png|560px]]
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<b>Exp 3</b>
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<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
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<td width = "40px">a</td><td width = "255px"><font color = red>STAT1</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
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<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
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[[Image:KAIST-exp3.png|560px]]
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<b>Exp 4</b>
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<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
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<td width = "40px">a</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">b</td><td width = "255px">Signal peptide</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">c</td><td width = "255px">Ig-like</td><td width = "200px">50*C</td><td width = "223px">60%</td><td width = "70px">339bp</td>
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<td width = "40px">d</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">e</td><td width = "255px">Antibody</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">f</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
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[[Image:KAIST-exp3.png|560px]]
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Revision as of 15:49, 12 September 2010

 

PCR Result

9/6 ~ 9/9 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT153*C--
bV_STAT153*C--
cFusion Antibody receptor53*C--
dSignal peptide56*C--
eIg-like56*C--
fSignal Peptide + Ig-like56*C--
gAntibody56*C--
hV_Fusion Antibody Receptor53*C--
iB_FGFR156*C--
jVB_FGFR156*C--



KAIST-exp1.png

Exp 2

SegmentAnnealing TemperatureResult
aSTAT148*C60%2139bp
bV_STAT148*C--



KAIST-exp2.png

Exp 3

SegmentAnnealing TemperatureResult
aSTAT150*C100%2139bp
bV_STAT150*C--



KAIST-exp3.png

Exp 4

SegmentAnnealing TemperatureResult
aFusion Antibody receptor50*C--
bSignal peptide50*C--
cIg-like50*C60%339bp
dSignal Peptide + Ig-like50*C--
eAntibody50*C--
fV_Fusion Antibody Receptor50*C--



KAIST-exp3.png


Retrieved from "http://2010.igem.org/Team:KAIST-Korea/Project/Methods/PCR_Result"