Team:KAIST-Korea/Project/Methods/PCR Result

From 2010.igem.org

(Difference between revisions)
(→9/6 ~ 9/9 PCR Result)
(→9/6 ~ 9/9 PCR Result)
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<td width = "400px"><b>Segment</b></td><td width = "400px"><b>Result</b></td>
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<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Temperature</b></td><td width = "300px"><b>Result</b></td>
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<td width = "40px">a</td><td width = "355px">STAT1</td><td width = "293px">-</td><td width = "100px">-</td>
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<td width = "40px">a</td><td width = "255px">STAT1</td><td width = "223px">-</td><td width = "70px">-</td>
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<td width = "40px">b</td><td width = "355px">V_STAT1</td><td width = "293px">-</td><td width = "100px">-</td>
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<td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "223px">-</td><td width = "70px">-</td>
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Revision as of 15:24, 12 September 2010

 

PCR Result

9/6 ~ 9/9 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



exp 1

SegmentTemperatureResult
aSTAT1--
bV_STAT1--
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