Team:KAIST-Korea/Project/Methods/PCR Result

From 2010.igem.org

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(→9/6 ~ 9/9 PCR Result)
(→9/6 ~ 9/9 PCR Result)
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==9/6 ~ 9/9 PCR Result==
==9/6 ~ 9/9 PCR Result==
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<b>PCR composition</b>
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<span style=font-size:12px><b>PCR composition </b></span>
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<table border=1>
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<br>
<br>
<b>exp 1</b>
<b>exp 1</b>
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<td><b>Segment</b></td><td><b>Result</b></td>
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<td>a</td><td>STAT1</td><td>-</td><td>-</td>
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<td>V_STAT_F</td><td>50pmole</td>
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</tr>
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<td>V_STAT_R</td><td>50pmole</td>
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</tr>
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<td>dNTP (dNTP mixture 2.5mM TaKaRa)</td><td>5ul</td>
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<td>Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)</td><td>0.25ul</td>
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</tr>
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<td>10x buffer (10x cloned Pfu Reaction buffer)</td><td>5ul</td>
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<td>DW (3rd sterile water)</td><td>rest</td>
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<td>Total</td><td>50ul</td>
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</tr>
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</table>

Revision as of 15:11, 12 September 2010

 

PCR Result

9/6 ~ 9/9 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



exp 1

SegmentResult
aSTAT1--
V_STAT_F50pmole
V_STAT_R50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)5ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul
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