Team:KAIST-Korea/Project/Methods/PCR Result

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(New page: __NOTOC__ {{:Team:KAIST/header}} <table width="100%" border="0" cellpadding = "20px"> <tr> <td valign="top"> <span style=font-size:30px><font face="Maiandra GD"><b>PCR Result</b></font>...)
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==9/6 ~ 9/9 PCR Result==
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<b>PCR composition</b>
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<table border=1>
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<tr align=center>
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<td><b>Materials</b></td><td><b>Amount</b></td>
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<td>Template</td><td>100pmole</td>
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<td>Primer A</td><td>50pmole</td>
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</tr>
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<td>Primer B</td><td>50pmole</td>
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<td>dNTP (dNTP mixture 2.5mM TaKaRa)</td><td>2ul</td>
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<td>Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)</td><td>0.25ul</td>
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<td>10x buffer (10x cloned Pfu Reaction buffer)</td><td>5ul</td>
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<td>DW (3rd sterile water)</td><td>rest</td>
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<td>Total</td><td>50ul</td>
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</table>

Revision as of 15:06, 12 September 2010

 

PCR Result

9/6 ~ 9/9 PCR Result

PCR composition


MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul
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