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Team:TU Delft/protocols/agarose gel
From 2010.igem.org
(Difference between revisions)
(New page: ==Agarose gel== 1. Dissolve 1 g of agarose in 100 mL 1x TBE buffer for 1% gel (can be stored in the 70 °C stove) 2. Add 5 µL of SYBRSafe (Invitrogen)) |
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| - | + | =Agarose gel= | |
| + | |||
| + | ''Material:'' | ||
| + | |||
| + | - agarose | ||
| + | |||
| + | - TBE buffer | ||
| + | |||
| + | - SYBRSafe (Invitrogen) | ||
| + | |||
| + | |||
| + | ''Protocol:'' | ||
1. Dissolve 1 g of agarose in 100 mL 1x TBE buffer for 1% gel (can be stored in the 70 °C stove) | 1. Dissolve 1 g of agarose in 100 mL 1x TBE buffer for 1% gel (can be stored in the 70 °C stove) | ||
| - | 2. Add 5 µL of SYBRSafe | + | 2. Add 5 µL of SYBRSafe |
Latest revision as of 11:09, 12 September 2010
Agarose gel
Material:
- agarose
- TBE buffer
- SYBRSafe (Invitrogen)
Protocol:
1. Dissolve 1 g of agarose in 100 mL 1x TBE buffer for 1% gel (can be stored in the 70 °C stove)
2. Add 5 µL of SYBRSafe
