Team:UC Davis/protocols/ligation.html

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Revision as of 22:27, 9 September 2010

Ligation

Materials

You will need:

  • Vector (plasmid) DNA sample
  • Insert DNA sample
  • Ligation buffer
  • DNA ligase
  • MilliQ water

Extra Notes

Procedure

Purpose

To ligate the desired insert fragment to the desired plasmid together.

References

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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