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Revision as of 14:58, 9 September 2010 (view source) Wishyx (Talk | contribs) (New page: == YX's part == Checked all the sequencing results before. The sequence was correct. Then the effect of the resistance gene must be checked.) ← Older edit		Latest revision as of 15:07, 9 September 2010 (view source) Wishyx (Talk | contribs) (→YX's part)
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	== YX's part ==		== YX's part ==
-	Checked all the sequencing results before. The sequence was correct.	+	Inoculated Chloramphenicol-LB with bacteria harboring Chloramphenicol resistance gene containing T-vectors. Inoculated the same media with bacteria harboring T-vectors with scrambled insertion sequence. Shook the media in a 37 centigrade shaker.
-	Then the effect of the resistance gene must be checked.	+	Checked the results after 13 hours. Both media became turbid. The one with scrambled sequence became darker in color, indicating possible contamination. Decided to repeat the experiments later.

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Latest revision as of 15:07, 9 September 2010

YX's part

Inoculated Chloramphenicol-LB with bacteria harboring Chloramphenicol resistance gene containing T-vectors. Inoculated the same media with bacteria harboring T-vectors with scrambled insertion sequence. Shook the media in a 37 centigrade shaker.

Checked the results after 13 hours. Both media became turbid. The one with scrambled sequence became darker in color, indicating possible contamination. Decided to repeat the experiments later.