Team:UNIPV-Pavia/Calendar/July

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Revision as of 08:56, 9 September 2010

JULY

Bio-Lab activity proceeds!

Select the day you are interested in to find out the details!

DATE	ACTIVITY
July, 1st	19° Bio-Lab - I7-3,4 and 5 and I8-3, 4 and 5 were prepared for TECAN test. Of the plates incubated yesterday, a colony was picked and grown in 1ml LB (+ antibiotic) for 6 hours at 37°C 220 rpm. Other parts were received from Anderson Lab.
July, 2nd	20° Bio-Lab - TECAN test provided encouraging results, showing that I7-3, I7-5, I8-4 and I8-5 produced GFP. Other culture (I7-1, I7-2, I7-4, I8-1, I8-2, I8-3) did not produce GFP, so they were thrown away. A furhter screening were performed on I7-3, I7-5, I8-4, I8-5 and this time all the clones are OK . Inoculum of <partinfo>BBa_J72007</partinfo> and <partinfo>BBa_J72013</partinfo> from LB agar plates.A glycerol stock was prepared for each culture. Inoculum of <partinfo>BBa_J72008</partinfo> from LB agar plate grown at 30°C 220 rpm overnight.
JULY: WEEK 2
July, 5th	21° Bio-Lab - LB agar plates prepared.
July, 6th	22° Bio-Lab - We decided to perform a TECAN test to evaluate the strength of some promoters belonging to the Anderson Promoters Collection.
July, 7th	23° Bio-Lab -Competent cells preparation for: * MG1655 * BW53474 * BW25141 * BW25142 PCR was performed to amplify Phasins. 11° Meeting - Updating about Bio-Lab activity.
July, 8th	24° Bio-Lab - Transformation of <partinfo>BBa_J23118</partinfo> in our home-made competent strains MG1655 BW25141 BW25142 BW53474 DH5alpha (as control) to check transformation efficiency.
July, 9th	25° Bio-Lab - All plates showed red colonies, so we calculated the efficiency of the strains.
JULY: WEEK 3
July, 12th	26° Bio-Lab -
July, 13th	27° Bio-Lab -
July, 14th	28° Bio-Lab -
July, 15th	29° Bio-Lab -
July, 16th	30° Bio-Lab -
JULY: WEEK 4
July, 19th	31° Bio-Lab -
July, 20th	32° Bio-Lab -
July, 21st	33° Bio-Lab -
July, 22nd	34° Bio-Lab -
July, 23rd	35° Bio-Lab -
July, 24th	36° Bio-Lab -
July, 25th	37° Bio-Lab -
JULY: WEEK 5
July, 26th	38° Bio-Lab - 12° Meeting - Updating about Bio-Lab activity and organization of our trip to Boston!
July, 27th	39° Bio-Lab -
July, 28th	40° Bio-Lab -
July, 29th	41° Bio-Lab -
July, 30th	42° Bio-Lab -
July, 31th	43° Bio-Lab -

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 </td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 6th|July, 6th]]</td><td align="left">
-° Bio-Lab -
+° Bio-Lab - We decided to perform a TECAN test to evaluate the strength of some promoters belonging to the Anderson Promoters Collection.
 </td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 7th|July, 7th]]</td><td align="left">
-° Bio-Lab - <br>
+° Bio-Lab -Competent cells preparation for:
+    * MG1655
+    * BW53474
+    * BW25141
+    * BW25142
+PCR was performed to amplify Phasins.
+<br>
 ° Meeting - Updating about Bio-Lab activity.
 </td></tr>