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Team:UNIPV-Pavia/Calendar/July
From 2010.igem.org
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<table class=cont border="0" width="30%" align="center" valign="top"> | <table class=cont border="0" width="30%" align="center" valign="top"> | ||
<tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr> | ||
| - | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #July, 1st|July, 1st]]</td><td align="left">19° Bio-Lab - | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #July, 1st|July, 1st]]</td><td align="left"> |
| - | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #July, 2nd|July, 2nd]]</td><td align="left">20° Bio-Lab - | + | 19° Bio-Lab -I7-3,4 and 5 and I8-3, 4 and 5 were prepared for TECAN test.<br> |
| + | Of the plates incubated yesterday, a colony was picked and grown in 1ml LB (+ antibiotic) for 6 hours at 37°C 220 rpm. | ||
| + | Other parts were received from Anderson Lab. | ||
| + | </td></tr> | ||
| + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #July, 2nd|July, 2nd]]</td><td align="left"> | ||
| + | 20° Bio-Lab - TECAN test provided encouraging results, showing that I7-3, I7-5, I8-4 and I8-5 produced GFP.<br> | ||
| + | Other culture (I7-1, I7-2, I7-4, I8-1, I8-2, I8-3) did not produce GFP, so they were thrown away. A furhter screening were performed on I7-3, I7-5, I8-4, I8-5 and this time all the clones are OK . | ||
| + | Inoculum of <partinfo>BBa_J72007</partinfo> and <partinfo>BBa_J72013</partinfo> from LB agar plates.A glycerol stock was prepared for each culture.<br> | ||
| + | Inoculum of <partinfo>BBa_J72008</partinfo> from LB agar plate grown at 30°C 220 rpm overnight. | ||
| + | </td></tr> | ||
<tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 2</b></th></tr> | <tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 2</b></th></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 5th|July, 5th]]</td><td align="left">21° Bio-Lab</td></tr> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana2 #July, 5th|July, 5th]]</td><td align="left">21° Bio-Lab</td></tr> | ||
Revision as of 08:42, 9 September 2010
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