Team:EPF Lausanne/Protocols

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Revision as of 18:35, 7 September 2010

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Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

Adapt pH
Agarose Gel
Agar plates production
Autoclave
Competence Asaia (Nadine)
Electrophoresis
Growing Asaia
Ligation
Measuring DNA concentration using NanoDrop
Miniprep: use Miniprep Kit
Optical Density (OD)
PCR Roche
PCR iProof
Purification: use Purification Kit
Transformation of competent cells
UV Imaging
Using the plate reader (Solange)
Digestion
Western Blot

Media Recipes

Syntax: convention if you want to upload a new protocol

When uploading a file it please rename it in the following way: 
 prot_yourProcedureName.yourExtension.

@@ Line 36: / Line 36: @@
 *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation]
 *[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
-* Miniprep \Rightarrow use Miniprep Kit
+* Miniprep: use Miniprep Kit
 *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)]
 *[https://static.igem.org/mediawiki/2010/5/54/Protocol_pcr_roche.pdf PCR Roche ]
 *[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR iProof]
-* Purification --> use Purification Kit
+* Purification: use Purification Kit
 *[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells]
 *[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging]