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Team:Washington/Gram Positive/Build
From 2010.igem.org
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=Mutate DNA= | =Mutate DNA= | ||
OUTLINE | OUTLINE | ||
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Order Olglionucleotides with the desired mutation | Order Olglionucleotides with the desired mutation | ||
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Create ssDNA of wild type gene | Create ssDNA of wild type gene | ||
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Allow Olglios to annele to the ssDNA | Allow Olglios to annele to the ssDNA | ||
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Use polymerase and DNTPs to synthesize the rest of the gene | Use polymerase and DNTPs to synthesize the rest of the gene | ||
Revision as of 16:59, 7 September 2010
Mutate DNA
OUTLINE
Order Olglionucleotides with the desired mutation
Create ssDNA of wild type gene
Allow Olglios to annele to the ssDNA
Use polymerase and DNTPs to synthesize the rest of the gene
