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Team:EPF Lausanne/Project parts
From 2010.igem.org
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The BB we have for the moment are: | The BB we have for the moment are: | ||
| - | + | *C3 + OriA | |
| - | + | *C3 + OriA + Kan | |
| - | + | *C3 + OriA + Tet | |
| - | + | *C3 + PromS + RBS | |
| - | + | *C3 + PromW + RBS | |
| - | + | *C3 + PromS + RBS + Immu | |
We are actually working on insert the others 2 proteins we want to express (p25, p28) on C3 and trying to finally ligate the BioBricks in the BaseVector containing the origin of replication of Asaia. | We are actually working on insert the others 2 proteins we want to express (p25, p28) on C3 and trying to finally ligate the BioBricks in the BaseVector containing the origin of replication of Asaia. | ||
| Line 37: | Line 37: | ||
Legend: | Legend: | ||
| - | OriA = Origin of replication for Asaia | + | *OriA = Origin of replication for Asaia |
| - | PromS = Strong Promoter | + | *PromS = Strong Promoter |
| - | Prom W = Weak Promoter | + | *Prom W = Weak Promoter |
| - | RBS = Ribosome Binding Site | + | *RBS = Ribosome Binding Site |
| - | Immu = immunotoxin | + | *Immu = immunotoxin |
= Test 2 = | = Test 2 = | ||
Revision as of 09:43, 6 September 2010
BioBricks
For the moment we have 6 BioBricks verified by sequencing and by electophoresis. Those BioBricks are in the vector pSB1C3 (friendly called C3) and are ready to be send to the registry!
The BB we have for the moment are:
- C3 + OriA
- C3 + OriA + Kan
- C3 + OriA + Tet
- C3 + PromS + RBS
- C3 + PromW + RBS
- C3 + PromS + RBS + Immu
We are actually working on insert the others 2 proteins we want to express (p25, p28) on C3 and trying to finally ligate the BioBricks in the BaseVector containing the origin of replication of Asaia.
Legend:
- OriA = Origin of replication for Asaia
- PromS = Strong Promoter
- Prom W = Weak Promoter
- RBS = Ribosome Binding Site
- Immu = immunotoxin
Test 2
asd
