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Team:Tsinghua/Notebook/15 August 2010
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(New page: == Module I, DT and Fan's part: == PCR and double digestion to detect. Double digestion system: H2O 8μl buffer tango 4μl plasmid 5μl EcoRI 1μl BamHI ...)
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(New page: == Module I, DT and Fan's part: == PCR and double digestion to detect. Double digestion system: H2O 8μl buffer tango 4μl plasmid 5μl EcoRI 1μl BamHI ...)
Newer edit →
Revision as of 10:22, 3 September 2010
Module I, DT and Fan's part:
PCR and double digestion to detect.
Double digestion system:
H2O 8μl buffer tango 4μl plasmid 5μl EcoRI 1μl BamHI 2μl Total 20μl
PCR system (SuperMix):
H2O 8.5μl primer1 0.5μl primer2 0.5μl template 0.5μl SuperMix 10μl Total 20μl
result
The result is quite strange. We’ll buy a pair of pUC19 universal primers to run the PCR. Also, we doubt that the PEM plasmids are not pure. So we transfer the plasmids PEM again to purify.
