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Team:Tsinghua/Notebook/13 July 2010
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(New page: == Module I, DT and Fan's part: == PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragmen...)
Newer edit →
(New page: == Module I, DT and Fan's part: == PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragmen...)
Newer edit →
Revision as of 09:03, 3 September 2010
Module I, DT and Fan's part:
PCR again with 35 cycles. Run a gel, cut and gel extract. We have improved our protocol for ligation, that is, ligate the first two and last two fragments first, if the concentrate is low, go through a PCR and link them.
