Team:Stockholm/31 August 2010

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(New page: {{Stockholm/Top2}} == Mimmi == === MITF-m === ==== Gel ==== Image:2010-08-31_pSB1C3.MITF-M_8-14_+_digested.jpg {| ! well ! sample |- | 1 | ladder |- | 2 | pSB1C3.M...)
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Revision as of 15:37, 31 August 2010


Contents

Mimmi

MITF-m

Gel

2010-08-31 pSB1C3.MITF-M 8-14 + digested.jpg
well sample
1 ladder
2 pSB1C3.MITF-M 8
3 pSB1C3.MITF-M 9
4 pSB1C3.MITF-M 10
5 pSB1C3.MITF-M 11
6 pSB1C3.MITF-M 12
7 pSB1C3.MITF-M 13
8 pSB1C3.MITF-M 14
9 positive control
10 digested MITF (E+S)
11 digested pSB1C3 (E+S)


Re-ligation and transformation

  • Depohosphorylate vector:
Mix Conditions
cut pSB1C3 9 time °C
A phosp 1 10m 37
tot 10µl 5m 75
oo 10


  • Ligate
Mix (µl) Conditions
cut pSB1C3 2 time °C
cut MITF-M 13 10m 22
5x buffer 4
T4 ligase 1
sH2O 0
tot 20µl


  • Follow the original transformation protocol