Team:Stockholm/31 August 2010
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(New page: {{Stockholm/Top2}} == Mimmi == === MITF-m === ==== Gel ==== Image:2010-08-31_pSB1C3.MITF-M_8-14_+_digested.jpg {| ! well ! sample |- | 1 | ladder |- | 2 | pSB1C3.M...)
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(New page: {{Stockholm/Top2}} == Mimmi == === MITF-m === ==== Gel ==== Image:2010-08-31_pSB1C3.MITF-M_8-14_+_digested.jpg {| ! well ! sample |- | 1 | ladder |- | 2 | pSB1C3.M...)
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Revision as of 15:37, 31 August 2010
Contents |
Mimmi
MITF-m
Gel
well | sample |
---|---|
1 | ladder |
2 | pSB1C3.MITF-M 8 |
3 | pSB1C3.MITF-M 9 |
4 | pSB1C3.MITF-M 10 |
5 | pSB1C3.MITF-M 11 |
6 | pSB1C3.MITF-M 12 |
7 | pSB1C3.MITF-M 13 |
8 | pSB1C3.MITF-M 14 |
9 | positive control |
10 | digested MITF (E+S) |
11 | digested pSB1C3 (E+S) |
Re-ligation and transformation
- Depohosphorylate vector:
Mix | Conditions | |||
---|---|---|---|---|
cut pSB1C3 | 9 | time | °C | |
A phosp | 1 | 10m | 37 | |
tot | 10µl | 5m | 75 | |
oo | 10 |
- Ligate
Mix | (µl) | Conditions | ||
---|---|---|---|---|
cut pSB1C3 | 2 | time | °C | |
cut MITF-M | 13 | 10m | 22 | |
5x buffer | 4 | |||
T4 ligase | 1 | |||
sH2O | 0 | |||
tot | 20µl |
- Follow the original transformation protocol