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Team:USTC/Notebook/Meetings/30 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: Transformation of ligation product:newly pduJK PCR product in PSB1C3 vector (XbaI/PstI)%>_<%use the wrong enzyme do it again!... Miniprep of pduJK-5 PCR of pduJ and pduK in new standard....) |
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Transformation of ligation product:newly pduJK PCR product in PSB1C3 vector (XbaI/PstI)%>_<%use the wrong enzyme do it again!... | Transformation of ligation product:newly pduJK PCR product in PSB1C3 vector (XbaI/PstI)%>_<%use the wrong enzyme do it again!... | ||
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PCR of pduJ and pduK in new standard.=> Restriction Enzyme Cutting.=> [in Aug.31st]ligation with PSB1C7. (earI)=> Transformation | PCR of pduJ and pduK in new standard.=> Restriction Enzyme Cutting.=> [in Aug.31st]ligation with PSB1C7. (earI)=> Transformation | ||
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Revision as of 17:00, 30 August 2010
Jocelyn:
Transformation of ligation product:newly pduJK PCR product in PSB1C3 vector (XbaI/PstI)%>_<%use the wrong enzyme do it again!...
Miniprep of pduJK-5
PCR of pduJ and pduK in new standard.=> Restriction Enzyme Cutting.=> [in Aug.31st]ligation with PSB1C7. (earI)=> Transformation
The end...
