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Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/25
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(New page: ==Experiment5：Extraction of a gel== ＜Member＞Mariko, rsk, hitomi ＜Materials＞<br /> ・Thermostable β-Agarase 6 units<br /> ・Agarose gel(after DNA gel electrophoresis)<br /> ...)
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(New page: ==Experiment5：Extraction of a gel== ＜Member＞Mariko, rsk, hitomi ＜Materials＞<br /> ・Thermostable β-Agarase 6 units<br /> ・Agarose gel(after DNA gel electrophoresis)<br /> ...)
Newer edit →
Revision as of 07:54, 25 August 2010
Experiment5：Extraction of a gel
＜Member＞Mariko, rsk, hitomi
＜Materials＞
・Thermostable β-Agarase 6 units
・Agarose gel(after DNA gel electrophoresis)
・Dropped water 400ml
＜Protocol＞
1 Prepared 400ml solution to contain Yeast extract 2.0g, Peptone 2.0g, Glucose 40.0g, NaCl 2.0g and Agerose 6.0g.
2 Divided up 400ml solution into two Erlenmeyer flasks, and sealed with aluminum. Afterwards , started autoclave(121℃,20min)
3 After finished autoclave, in cleanbench, added 0.2ml Ampcillm to each of the solutions. Afterwards, divided solutions into twenty Petri dishes.
4 Cooled and dried divided solutions in Petri dishes. After Cooling and drying well, kept these in refrigerator.
