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Team:Michigan/Protocols
From 2010.igem.org
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[[Media:7-31-2010_Ag43_flu_gene_WITH_MODIFIED_PRIMER_SITES.pdf|Detailed primer design]] | [[Media:7-31-2010_Ag43_flu_gene_WITH_MODIFIED_PRIMER_SITES.pdf|Detailed primer design]] | ||
[[Media:8-4-2010_Colony_PCR_flu.pdf|colony PCR protocol]] | [[Media:8-4-2010_Colony_PCR_flu.pdf|colony PCR protocol]] | ||
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| + | ====Pili==== | ||
| + | [[Media:FimB_Ligation_Protocol.pdf|FimB Ligation]] | ||
====Quorum Sensing==== | ====Quorum Sensing==== | ||
Revision as of 22:43, 23 August 2010
Protocols
Using Lab Equipment
Obtaining Deionized Water in the ERB
Enigeering Research Building Autoclave
Epifluorescence Microscope Usage - H.H. Dow
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
DNA Manipulation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Heat shock
Transformation-electroporation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
Updated T4 DNA Ligase Protocol (Not quick ligase)
- Ligation
- Ginko Bioworks Protocol
- Jeremy Minty Protocol
Group-Specific Protocols
Oil Sands
Revised biofilm assay protocol
Detailed primer design colony PCR protocol
Pili
Quorum Sensing
General Outline for Biobrick Manipulations
