"
Team:Stockholm/20 August 2010
From 2010.igem.org
(Difference between revisions)
m |
(→Hassan) |
||
| Line 6: | Line 6: | ||
midnight update, after a fast recovery from sickness! | midnight update, after a fast recovery from sickness! | ||
| + | ===Version 0.5.1=== | ||
<html> | <html> | ||
<embed | <embed | ||
| Line 16: | Line 17: | ||
</html> | </html> | ||
| - | + | ===Version 0.6.1=== | |
| - | + | <html> | |
| - | + | <embed | |
| + | src="https://static.igem.org/mediawiki/2010/c/cb/Test_SU_vitiligo_animation_0_6_1.swf" | ||
| + | width="800" | ||
| + | height="400" | ||
| + | allowscriptaccess="always" | ||
| + | allowfullscreen="true" | ||
| + | /> | ||
| + | </html> | ||
== Mimmi == | == Mimmi == | ||
Revision as of 19:36, 20 August 2010
| Home | Project Idea | Lab Work | Results | Modelling | Team | Follow us on  and 
| 2010.igem.org 
|
Contents |
Hassan
midnight update, after a fast recovery from sickness!
Version 0.5.1
Version 0.6.1
Mimmi
pMA
Primer dilution
| pMA_VF | 91µl sH2O --> 100µM | 10µl + 90µl sH2O --> 10µM | ||
| pMA_VR | 91µl sH2O --> 100µM | 10µl + 90µl sH2O --> 10µM |
pMA/pEX
verification PCR
| pEX | pMA | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Mix | (µl) | X6 | X3 | primers | conditions | ||||
| sH2O | 22.5 | 135 | 67.5 | pEX_VF | time | °C | |||
| F primer | 1 | 6 | 3 | pEX_VR | 2m | 94 | |||
| R primer | 1 | 6 | 3 | pMA_VF | 30s | 94 | ) | ||
| DNA | 0.5 | 5X0.5 | 2X0.5 | pMA_VR | 30s | 60 | > 30 cycles | ||
| tot | 25µl | 250µl | 75µl | 2m30s | 72 | ) | |||
| 10m | 72 | ||||||||
| oo | 10 | ||||||||
