Team:Michigan/Project
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== '''Overall project''' == | == '''Overall project''' == | ||
- | Our project | + | Our team decided to work on two projects simultaneously this year. The first involves making an efficient microalgae bioflocculant out of ''E. coli'' to improve oil harvesting from algae. For this project, we will be over-expressing type I pili and their associated adhesion protein, making a hyper-piliated, hyper-adhesive strain of ''E. coli''. We hope that the extreme stickiness of the ''E. coli'' will turn them into an excellent bioflocculant- termed EcoGlue. In addition, we will also be expressing a virus protein which specifically binds to the algal species ''Chlorella vulgaris'', a species of interest for harvesting algae oil. This protein will provide additional flocculation ability and specificity for this species. We hope that such a bioflocculant can provide a cheaper, safer alternative to chemical flocculants. |
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+ | Our second project is working on the oil sands initiative, where we will be trying to reduce the reclamation time of tailings water, which must generally be retained for over 10 years. These waters are tainted with toxic naphthenic acids (NAs), and we will be trying to indirectly improve the natural degradation abilities of two ''Pseudomonas'' species, which were previously isolated from tailings pond sediment and found to degrade NAs. Since little to nothing is known about the degradation pathway itself, it is difficult to manipulate the pathway, however, these two species have a synergistic metabolism, and the efficiency of degradation is drastically increased when they are co-cultured. So we will be introducing a self-dimerizing surface protein into the two species, in order to ensure that they stick together, and also potentially increase their flocculation abilities, as we have seen that Pseudomonas to not make biofilms at the high pH levels seen in tailings waters. We want these bacteria to be in a biofilm in hopes that it will increase the efficiency and rate of their NA degradation. We will also be cloning a gene into both species that triggers dense, thick biofilms when over-expressed. This gene also prevents biofilm dispersal under carbon starvation, ensuring that a biofilm will stick around in one place even as NA levels diminish, ensuring degradation to the lowest level possible. | ||
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== Pili Hyperexpression == | == Pili Hyperexpression == |
Revision as of 15:35, 20 August 2010