Team:Calgary/25 May 2010

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(New page: '''Tuesday May 25, 2010''' Today, we observed our plates where competent cells with our constructs were grown and observed for RFP under a UV light. We also held a discussion on what we...)
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'''Tuesday May 25, 2010'''
'''Tuesday May 25, 2010'''
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Today, we observed  our plates where competent  cells with our constructs were grown and observed for RFP under a UV light. We also held a discussion on what we could do with the ethics and judging portion of our project. In addition to this, we discussed possible community outreach that we could do to promote synthetic biology. We made antibiotic broth and attempted construction again using the same plasmids that did not contain the DNA binding dye.
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Today, we opened the DNA that was sent from the Registry of Parts. We attempted rehydration of plasmids pSB1A2 and pSB2K3 and then using that DNA, we attempted a construction of RFP using J13002 and E1010. We did restriction digest using the protocols indicated and then attempted the construction. The constructions were then plated and then left to grow.

Revision as of 22:07, 28 May 2010


Tuesday May 25, 2010

Today, we opened the DNA that was sent from the Registry of Parts. We attempted rehydration of plasmids pSB1A2 and pSB2K3 and then using that DNA, we attempted a construction of RFP using J13002 and E1010. We did restriction digest using the protocols indicated and then attempted the construction. The constructions were then plated and then left to grow.

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