Team:UNIPV-Pavia/Calendar/August/settimana3

From 2010.igem.org

(Difference between revisions)
(August, 17th)
(August, 17th)
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Pick of <partinfo>BBa_J13002</partinfo>, <partinfo>BBa_I13522</partinfo>, INTEIN, CREAM, co-trasformed <partinfo>BBa_T9002</partinfo> and inoculum into 1 ml of LB+Amp, LB+Kan, LB+Cm, respectively, to make glycerol stocks. Falcons were than refilled for the minipreps of the following day.
Pick of <partinfo>BBa_J13002</partinfo>, <partinfo>BBa_I13522</partinfo>, INTEIN, CREAM, co-trasformed <partinfo>BBa_T9002</partinfo> and inoculum into 1 ml of LB+Amp, LB+Kan, LB+Cm, respectively, to make glycerol stocks. Falcons were than refilled for the minipreps of the following day.
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----
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Cultures were diluted (5ul in 2ml LB+antibiotic) for TECAN test:
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{| border='1' align='center'
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|I7-A || I8-5 D|| I9-1
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|-
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|I10-B|| I12-2|| A2
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|-
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|<partinfo>BBa_B0032</partinfo>|| I144C5-T9002|| I164C5-T9002
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|-
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|I174C5-T9002|| I184C5-T9002|| I194C5-T9002
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|-
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|ENTERO4C5-T9002|| || ||
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|}
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 +
Glycerol stock was prepared for ENTERO4C5-T9002.
==August, 18th==
==August, 18th==

Revision as of 09:12, 19 August 2010


AUGUST: WEEK 3



August, 16th

Resuspension from iGEM 2010 Distribution Kit of <partinfo>BBa_J13002</partinfo> (Plate 1, Well 13B) and <partinfo>BBa_I13522</partinfo> (Plate 2, Well 8A).

Transformation (1 ul) of <partinfo>BBa_J13002</partinfo>, <partinfo>BBa_I13522</partinfo>, INTEIN, CREAM (Mr.Gene) into 100 ul of E. coli TOP10 home-made competent cells.

Transformation of 15-4C5 (1 ul) into in re-competentized E. coli TOP10 carrying <partinfo>BBa_T9002</partinfo>.

Transfomants were plated on LB+Amp 100 ug/ml agar plates, the last one on LB+Amp100+Cm12,5 agar plates and were let grow ON at 37°C.

August, 17th

All plates showed colonies.

Pick of <partinfo>BBa_J13002</partinfo>, <partinfo>BBa_I13522</partinfo>, INTEIN, CREAM, co-trasformed <partinfo>BBa_T9002</partinfo> and inoculum into 1 ml of LB+Amp, LB+Kan, LB+Cm, respectively, to make glycerol stocks. Falcons were than refilled for the minipreps of the following day.


Cultures were diluted (5ul in 2ml LB+antibiotic) for TECAN test:

I7-A I8-5 D I9-1
I10-B I12-2 A2
<partinfo>BBa_B0032</partinfo> I144C5-T9002 I164C5-T9002
I174C5-T9002 I184C5-T9002 I194C5-T9002
ENTERO4C5-T9002

Glycerol stock was prepared for ENTERO4C5-T9002.

August, 18th

August, 19th

August, 20th

August, 21st

August, 22nd