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Team:LMU-Munich/Cut'N'survive/Schedule/PCR key
From 2010.igem.org
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Revision as of 15:10, 18 August 2010
1TreF 56°C:
*Number: consecutive numbering on PCR plan
*Tre: Abbreviation of gene of interest
*F: Forward Primer
*56°C: Annealing temperature (4+2 rule)
4TevMutEPR :
*Mut: Mutagenesis Primer
*E: EcoRI digestion site
*P: PstI digestion site
*R: Reverse Primer
Also:
*S: SpeI digestion site
Primer
Primer overhang with Biobrick digestion sites
Digestion site which is to be mutated
Template
