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Team:LMU-Munich/Jump-or-die/Schedule
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==Aim 1: DNA reproduction+PCR== | ==Aim 1: DNA reproduction+PCR== | ||
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| + | For PCR key see [[Team:LMU-Munich/Cut'N'survive/Schedule/PCR key | PCR key]] | ||
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<b>Colourcode for the primers: <font color="#FF0000">Annealing part</font>, <font color="#009933">mutagenised part</font>, <font color="#FF6600">other sequences integrated into primer</font></b> | <b>Colourcode for the primers: <font color="#FF0000">Annealing part</font>, <font color="#009933">mutagenised part</font>, <font color="#FF6600">other sequences integrated into primer</font></b> | ||
Revision as of 14:44, 18 August 2010
For PCR key see PCR key
Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer
PCR1: replication of the Tet-inducible CMV promotor [X]
PCR4a: replication human bak with mutagenesis (Pst1) [ ]
PCR4b: replication human bak with mutagenesis (Pst1) [ ]
PCR5: joining PCR of human bak [ ]
PCR6: replication of the SV40-polyadenylation site [X]
PCR9: replication of eGFP with attP in primer [ ]
Primer used: 20eGFPattPF 21eGFPR
PCR10: Replikation of PhiC31o [ ]
Primer used: 22PC31oF 23PC31oR
Note: for attB two Primers ( 18attBF 19attBR) used.
Contents
Aim 1: DNA reproduction+PCR
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
