Team:LMU-Munich/Cut'N'survive/Schedule
From 2010.igem.org
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(→Aim 1: DNA reproduction+PCR) |
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==Aim 1: DNA reproduction+PCR== | ==Aim 1: DNA reproduction+PCR== | ||
+ | PCR1: replication of the Tet-inducible CMV promotor | ||
+ | PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) | ||
+ | PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) | ||
+ | PCR3: joining PCR of the TEVrecogn-NDegron-SF3 Part | ||
+ | PCR4a: replication human bak with mutagenisis (Pst1) | ||
+ | PCR4b: replication human bak with mutagenisis (Pst1) | ||
+ | PCR5: joining PCR of human bak | ||
+ | PCR6: replication of the SV40-polyadenylation site | ||
+ | PCR7a: replication of the p14*TEVrecogn with mutagenisis (Spe1) | ||
+ | PCR7b: replication of the p14*TEVrecogn with mutagenisis (Spe1) | ||
+ | PCR8: joining PCR of p14*TEVrecogn | ||
+ | |||
==Aim 2: Assembling Biobricks== | ==Aim 2: Assembling Biobricks== | ||
==Aim 3: Testing products== | ==Aim 3: Testing products== |
Revision as of 10:30, 18 August 2010