Team:LMU-Munich/TEV-System/Schedule/Aim 1

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== Lab-Notebook ==
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Identification, multiplication and extraction of received plasmids.
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This notebook is used to structure our work and progress
 
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== DNA-multiplication ==
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CMV (= CMV-promoter, BBa_J52034)
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It is necessary to increase the amount of DNA we have got in order to work properly with it.
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pDS7 (= TEV recogn N Degron SF3b155)
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=== CMV-Promoter from BBJ52034 ===
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PhiC31o
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====Work====
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We have to proliferate E. colis containing this plasmid and then extract the plasmid from the bacteria.
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====Progress====
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8/9+10 Proliferation done by Corinna, Laura, Alex, Julia
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190-6 (= TEV p14 recogn)
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8/11 Planned extraction by Jara, Emanuel
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eGFP (= BBa_I714891)
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=== eGFP ===
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bak (= human bak)
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This Biobrick is needed several times.
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====Work====
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It has to be ...(?)
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====Progress====
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==PCR-steps==
 
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..
 
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===mutagenesis===
 
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...
 
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===fusion===
 
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...
 
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==cloning==
 
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fusions
 
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==insert in eukaryotic genom==
 
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if there is time left
 
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Latest revision as of 18:20, 17 August 2010


Identification, multiplication and extraction of received plasmids.


CMV (= CMV-promoter, BBa_J52034)

pDS7 (= TEV recogn N Degron SF3b155)

PhiC31o

190-6 (= TEV p14 recogn)

eGFP (= BBa_I714891)

bak (= human bak)



 

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