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Team:UNIPV-Pavia/Calendar/August/settimana3
From 2010.igem.org
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Resuspension from iGEM 2010 Distribution Kit of <partinfo>BBa_J13002</partinfo> (Plate 1, Well 13B) and <partinfo>BBa_I13522</partinfo> (Plate 2, Well 8A). | Resuspension from iGEM 2010 Distribution Kit of <partinfo>BBa_J13002</partinfo> (Plate 1, Well 13B) and <partinfo>BBa_I13522</partinfo> (Plate 2, Well 8A). | ||
| - | Transformation (1 ul) of <partinfo>BBa_J13002</partinfo>, <partinfo>BBa_I13522</partinfo>, INTEIN, CREAM (Mr.Gene) into 100 ul of TOP10 home-made competent cells. | + | Transformation (1 ul) of <partinfo>BBa_J13002</partinfo>, <partinfo>BBa_I13522</partinfo>, INTEIN, CREAM (Mr.Gene) into 100 ul of ''E. coli'' TOP10 home-made competent cells. |
| - | Transformation of 15-4C5 (1 ul) into in | + | Transformation of 15-4C5 (1 ul) into in re-competentized ''E. coli'' TOP10 carrying <partinfo>BBa_T9002</partinfo>. |
Transfomants were plated on LB+Amp 100 ug/ml agar plates, the last one on LB+Amp100+Cm12,5 agar plates and were let grow ON at 37°C. | Transfomants were plated on LB+Amp 100 ug/ml agar plates, the last one on LB+Amp100+Cm12,5 agar plates and were let grow ON at 37°C. | ||
Revision as of 15:09, 16 August 2010
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