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Team:RMIT Australia/Notebook
From 2010.igem.org
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<div id=" .2F3_August_2010">'''3 August''' | <div id=" .2F3_August_2010">'''3 August''' | ||
| - | Had iGEM team meeting</div> | + | Had iGEM team meeting |
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| + | -Discussed ANZAAS Proposal | ||
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| + | -Spoke about possibilities of using Geneart | ||
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| + | -Discussed possible T-shirt designs | ||
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| + | </div> | ||
<div id=" .2F10_August_2010">'''10 August''' | <div id=" .2F10_August_2010">'''10 August''' | ||
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Had iGEM team meeting | Had iGEM team meeting | ||
| - | Took Team Photo | + | -Took Team Photo |
</div> | </div> | ||
| + | <div id=" .2F11_August_2010">'''11 August''' | ||
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| + | PCR of Taq | ||
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| + | Taq with a DNA concentration of 40ng/µL was diluted to 1µM by addition of 24µL of MilliQ water to 1µL of the Taq plasmid. Primers were diluted to 100µM by adding 354µL to the forward primer with a concentration of 35.4 nmol and 405µL to the reverser primer with a concentration of 40.5nmol. The primers were further diluted to 0.2µM by adding 10µL of the primer to 490µL of milliQ water. The PCR reaction was then made by adding 25µL of Master Mix, 2.5µL of each primer, 5µL of Rnase free water and 15µL of 0.2µM Taq Plasmid. | ||
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| + | The PCR | ||
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| + | 98º 60 Sec 1 cycle | ||
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| + | 98º 15 Sec then 72º 90 Sec 25 cycle | ||
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| + | 72º 5 min 1 cycle | ||
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|} | |} | ||
Revision as of 06:19, 16 August 2010
Notebook
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