Team:Stockholm/9 August 2010

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(Difference between revisions)
(→Hassan)
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I think by end of this week a wall of text will appear in modelling page!
I think by end of this week a wall of text will appear in modelling page!
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== Mimmi ==
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=== MITF ===
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==== - Gel ====
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*No products!
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:--> Trying more cycles of low annealing temperature
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=== MITF + yCCS and RFP ===
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==== Verification PCR ====
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{|
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! Mix
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| (µl)
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| X5
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| X3
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| rowspan="13" width="150" |
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! colspan="2" | '''yCCS/RFP'''
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| rowspan="8" width="150" |
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! colspan="2" | '''MITF'''
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| rowspan="8" width="150" |
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|-
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| sH<sub>2</sub>O
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| 22.5
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| 112.5
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| 67.5
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| colspan="2" | pSB1_VF2
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| colspan="2" | MITF_F
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|-
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| F primer
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| 1
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| 5
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| 3
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| colspan="2" | pSB1_VR
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| colspan="2" | MITF_R
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|-
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| R primer
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| 1
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| 5
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| 3
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| colspan="2" | 970/925bp
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| colspan="2" | ~1300bp
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|-
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| DNA
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| 0.5
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| 4X0.5
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| 2X0.5
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| colspan="2" |
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| colspan="2" |
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|-
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| align="right" | tot
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| 25
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| colspan="2" |
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! colspan="2" | conditions
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! colspan="2" | conditions
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|-
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| colspan="4" rowspan="7" |
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! time
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! &deg;C
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! time
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! &deg;C
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|-
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| 2m
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| 94
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| 2m
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| 94
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|-
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| 30s
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| 94
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| )
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| 30s
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| 94
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| )
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|-
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| 30s
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| 60
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| > 30 cycles
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| 30s
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| 60
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| > 30 cycles
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|-
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| 2m
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| 72
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| )
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| 2m40s
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| 72
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| )
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|-
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| 10m
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| 72
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| rowspan="2" |
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| 10m
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| 72
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| rowspan="2" |
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|-
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| oo
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| 10
 +
| oo
 +
| 10
 +
|}

Revision as of 20:15, 10 August 2010

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Contents


Hassan

Folate

below links and articles are meant to be study when I'm finished with interaction map and prove of concept!

  • [http://www.ncbi.nlm.nih.gov/pubmed/12761857]
  • [http://www.ncbi.nlm.nih.gov/pubmed/17307853,18403248,17698004,17220358,16359333,10201405,15489424,2254281,15865426,17923103,11041843,2185835,11371182,9287033,1958664,8208138,8019142,8408018,8634297,1098865?report=docsum]
  • [http://www.ncbi.nlm.nih.gov/pubmed/15611104]
  • [http://www.ncbi.nlm.nih.gov/pubmed/17698004]
  • [http://www.ncbi.nlm.nih.gov/pubmed/8208138]
  • [http://www.genome.jp/kegg-bin/show_pathway?org_name=eco&mapno=00790&mapscale=&show_description=hide]

Possible Genes

  • decrease in CAT (Catalase) : [http://www.ncbi.nlm.nih.gov/pubmed/10537016][http://onlinelibrary.wiley.com/doi/10.1034/j.1600-0749.2003.00087.x/abstract]
  • increase in SOD (SOD3) : [http://www.ncbi.nlm.nih.gov/pubmed/12692376]
  • possible genes involved in Melanocytes adhesion: "An imbalance in cell adhesion molecules was also observed in vitiligo melanocytes. Differentially downregulated genes in vitiligo melanocytes included integrin alpha 7, integrin beta-like 1, integrin beta 7 and catenin alpha-like 1 (cadherin-associated protein). The adhesion between the melanocytes and the basal membrane is mediated by integrins and the contacts between melanocytes and keratinocytes are mediated by cadherins (Hara et al., 1994; Tang et al., 1994; Zambruno et al., 1993). The importance of regulated expression levels of cadherins have also been shown during migration of melanoblasts (Nishimura et al., 1999). It is difficult to predict if this specific downregulation of adhesion molecules impairs the attachment of the melanocytes in the basal layer of the epidermis; however, detachment and transepidermal loss of melanocytes have previously been reported in vitiligo vulgaris (Gauthier et al., 2003)."[http://www.ncbi.nlm.nih.gov/pubmed/18426409] May also look at [http://www.ncbi.nlm.nih.gov/pubmed/17850512] if more interested.


And those from July 22 with data from planning will bring enough explanation for interaction map.

I think by end of this week a wall of text will appear in modelling page!




Mimmi

MITF

- Gel

  • No products!
--> Trying more cycles of low annealing temperature


MITF + yCCS and RFP

Verification PCR

Mix (µl) X5 X3 yCCS/RFP MITF
sH2O 22.5 112.5 67.5 pSB1_VF2 MITF_F
F primer 1 5 3 pSB1_VR MITF_R
R primer 1 5 3 970/925bp ~1300bp
DNA 0.5 4X0.5 2X0.5
tot 25 conditions conditions
time °C time °C
2m 94 2m 94
30s 94 ) 30s 94 )
30s 60 > 30 cycles 30s 60 > 30 cycles
2m 72 ) 2m40s 72 )
10m 72 10m 72
oo 10 oo 10
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