Team:Stockholm/5 August 2010

From 2010.igem.org

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=== MITF-PAX3 ===
=== MITF-PAX3 ===
"...Pax3 simultaneously functions to initiate a melanogenic cascade while acting downstream to prevent terminal differentiation. Pax3 activates expression of Mitf, a transcription factor critical for melanogenesis while at the same time it competes with Mitf for occupancy of an enhancer required for expression of dopachrome tautomerase, an enzyme that functions in melanin synthesis." [http://www.ncbi.nlm.nih.gov/pubmed/15729346]
"...Pax3 simultaneously functions to initiate a melanogenic cascade while acting downstream to prevent terminal differentiation. Pax3 activates expression of Mitf, a transcription factor critical for melanogenesis while at the same time it competes with Mitf for occupancy of an enhancer required for expression of dopachrome tautomerase, an enzyme that functions in melanin synthesis." [http://www.ncbi.nlm.nih.gov/pubmed/15729346]
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== Mimmi ==
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=== MITF ===
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==== - Gel ====
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*No products!
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:--> Trying a different polymerase
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==== - amplifying and moving ====
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{|
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! Mix
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| (µl)
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| X18
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| rowspan="10" width="100" |
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! primers
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| rowspan="10" width="100" |
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!colspan="2" | conditions
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| rowspan="3"  |
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|-
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| sH<sub>2</sub>O
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| 22
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| 396
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| MITF_F
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! time
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! &deg;C
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|-
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| F primer
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| 1
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| 18
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| MITF_R
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| 2m
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| 94
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|-
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| R primer
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| 1
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| 18
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| rowspan="7" |
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| 30s
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| 94
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| )
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|-
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| DNA
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| 1
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| 18
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| 30s
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| 45, 51, 55
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| > 5 cycles
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|-
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| align="right" | tot
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| 25
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| 450
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| 3m
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| 72
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| )
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|-
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| colspan="3" rowspan="4" |
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| 30s
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| 94
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|    \
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|-
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| 3m
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| 72
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|    / 25 cycles
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|-
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| 10m
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| 72
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| rowspan="2"
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|-
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| oo
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| 10
 +
|}

Revision as of 20:12, 10 August 2010

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Contents

Andreas

No lab work.

Team logotype

Worked on the team logotype most of the day. I have made a few different versions to be voted for.

Hassan

MITF-PAX3

"...Pax3 simultaneously functions to initiate a melanogenic cascade while acting downstream to prevent terminal differentiation. Pax3 activates expression of Mitf, a transcription factor critical for melanogenesis while at the same time it competes with Mitf for occupancy of an enhancer required for expression of dopachrome tautomerase, an enzyme that functions in melanin synthesis." [http://www.ncbi.nlm.nih.gov/pubmed/15729346]





Mimmi

MITF

- Gel

  • No products!
--> Trying a different polymerase


- amplifying and moving

Mix (µl) X18 primers conditions
sH2O 22 396 MITF_F time °C
F primer 1 18 MITF_R 2m 94
R primer 1 18 30s 94 )
DNA 1 18 30s 45, 51, 55 > 5 cycles
tot 25 450 3m 72 )
30s 94 \
3m 72 / 25 cycles
10m 72 rowspan="2"
oo 10
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