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Team:Cambridge/Notebook/Week3
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Today we had another meeting in the morning to try and distribute jobs. Theo created a plan for an <html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K325000">LRE Biobrick</a></html> and submitted it to [https://www.dna20.com/ DNA 2.0] for a quote - we really wanted to get our DNA synthesised as quickly as possible. | Today we had another meeting in the morning to try and distribute jobs. Theo created a plan for an <html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K325000">LRE Biobrick</a></html> and submitted it to [https://www.dna20.com/ DNA 2.0] for a quote - we really wanted to get our DNA synthesised as quickly as possible. | ||
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Anja, Hannah and Emily had fun writing a draft article for [http://www.sterilin.co.uk/ Sterilin] describing what iGEM is, what we were hoping to accomplish and what Sterilin products we had been using. Peter and Will also created primer sequences for our first experiment. Quite a productive day! | Anja, Hannah and Emily had fun writing a draft article for [http://www.sterilin.co.uk/ Sterilin] describing what iGEM is, what we were hoping to accomplish and what Sterilin products we had been using. Peter and Will also created primer sequences for our first experiment. Quite a productive day! | ||
Revision as of 10:07, 10 August 2010
Notebook: Week 3
Week 3: Monday 26th July - Sunday 1st August
Monday
Today we had another meeting in the morning to try and distribute jobs. Theo created a plan for an LRE Biobrick and submitted it to DNA 2.0 for a quote - we really wanted to get our DNA synthesised as quickly as possible.
Anja, Hannah and Emily had fun writing a draft article for [http://www.sterilin.co.uk/ Sterilin] describing what iGEM is, what we were hoping to accomplish and what Sterilin products we had been using. Peter and Will also created primer sequences for our first experiment. Quite a productive day!
Tuesday
- Duncan provided several E. coli strains. We plated out TOP10 to create colonies from which to create competent cells for transformation.
- We organised all the stock in the lab
- We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
Wednesday
Inoculated a broth with Top 10. Making competent cells tomorrow.
Thursday
To do: prepare competent cells
Friday
- Preparing order for sythesis
- Theo aligned 300 luciferase-sequences to visualise consensus
- Peter and Theo discovered DINAmelt, downloaded UNA Fold to model Rcd dynamics
- Anja, Emily and Bill prepared chemically competent cells!
- By spending an hour in a cold room. It was cold but worth it. We got to drink hot chocolate.
Saturday
- Fernan tested our competent cells for us and they worked!
Sunday
- Anja, Bill, Emily and Theo discussed a final template for the wiki - it is looking pretty.
