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Team:Cambridge/Notebook/Week3
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{{:Team:Cambridge/Templates/headerbar|colour=#fad72a|title=Notebook: Week 3}} | {{:Team:Cambridge/Templates/headerbar|colour=#fad72a|title=Notebook: Week 3}} | ||
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| + | <div align="center">Week 3: Monday 26th July - Sunday 1st August </div> | ||
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| - | ==Monday== | + | == Monday== |
| + | {{:Team:Cambridge/Templates/rightpic|src=CamNoteWk3_1.jpg}} | ||
===Afternoon=== | ===Afternoon=== | ||
* Theo created a plan for an <html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K325000">LRE Biobrick</a></html>, and submitted it to DNA 2.0 for a quote. | * Theo created a plan for an <html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K325000">LRE Biobrick</a></html>, and submitted it to DNA 2.0 for a quote. | ||
* Anja, Hannah and Emily worked on text for Sterilin | * Anja, Hannah and Emily worked on text for Sterilin | ||
| - | * Peter and Will created primer sequences for first experiment | + | * Peter and Will created primer sequences for our first experiment |
==Tuesday== | ==Tuesday== | ||
Revision as of 09:15, 10 August 2010
Notebook: Week 3
Week 3: Monday 26th July - Sunday 1st August
Monday
Afternoon
- Theo created a plan for an LRE Biobrick, and submitted it to DNA 2.0 for a quote.
- Anja, Hannah and Emily worked on text for Sterilin
- Peter and Will created primer sequences for our first experiment
Tuesday
- Duncan provided several E. coli strains. We plated out TOP10 to create colonies from which to create competent cells for transformation.
- We organised all the stock in the lab
- We met with Dr. Summers to discuss quiescence. He was very encouraging but have yet to settle IP concerns. He also suggested considering mopping up Rcd from a leaky inducible promoter with antisense RNA.
Wednesday
Inoculated a broth with Top 10. Making competent cells tomorrow.
Thursday
To do: prepare competent cells
Friday
- Preparing order for sythesis
- Theo aligned 300 luciferase-sequences to visualise consensus
- Peter and Theo discovered DINAmelt, downloaded UNA Fold to model Rcd dynamics
- Anja, Emily and Bill prepared chemically competent cells!
- By spending an hour in a cold room. It was cold but worth it. We got to drink hot chocolate.
Saturday
- Fernan tested our competent cells for us and they worked!
Sunday
- Anja, Bill, Emily and Theo discussed a final template for the wiki - it is looking pretty.
