Team:UNIPV-Pavia/Calendar/August/settimana2

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|'''I21-new:'''||I21-1||I21-2||I21-3||I21-4||I21-5||I21-6||I21-7||I21-8||I21-9||I21-10||I21-11||I21-12
|'''I21-new:'''||I21-1||I21-2||I21-3||I21-4||I21-5||I21-6||I21-7||I21-8||I21-9||I21-10||I21-11||I21-12
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In order not to loose our time we decided to perform again the PCR-amplification/modification of <partinfo>BBa_K208001</partinfo> in case of a negative screening.
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Revision as of 10:35, 9 August 2010
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AUGUST: WEEK 2


August, 9th

Phasins plates showed very few colonies (17 for I20-new and 12 for I21-new): they were all picked and let grow in LB+Amp 100ug/ml. In the evening we made glycerol stocks and re-filled the tubes for the screening of the following day:

I20-new:I20-1I20-2I20-3I20-4I20-5I20-6I20-7I20-8I20-9I20-10I20-11I20-12I20-13I20-14I20-15I20-16I20-17
I21-new:I21-1I21-2I21-3I21-4I21-5I21-6I21-7I21-8I21-9I21-10I21-11I21-12

In order not to loose our time we decided to perform again the PCR-amplification/modification of <partinfo>BBa_K208001</partinfo> in case of a negative screening.

Check of LB+Cm 6 ug/ml agar plates. We let grow at 30°C:

  • MG1655 in LB
  • MC1061 in LB
  • MG123/008 in LB+Amp 50 ug/ml
  • MC123/008 in LB+Amp 50 ug/ml

Than we streaked cultures on plates. Since 6 ug/ml is a very low concentration, we wanted to check if actually nothing (without the right resistance) grow on these plates.

August, 10th

August, 11th

August, 12th

August, 13th

August, 14th

August, 15th



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